Abstract
A method for simultaneous concentration of multiple bioactive components (MBCs) in Danggui-Buxue Decoction (DBD) was comprehensively investigated in this work. The column chromatography with macroporous resin ADS-7 as an absorbent was used for concentration of the MBCs in DBD under the guidance of liquid chromatography (LC). After one-run treatment with the resin, the contents of major flavonoids reached 18.3 and 13.9% in the eluting fraction of 50 and 70% ethanol, which were 177.2 and 134.6 times higher than those in DBD crude extract. The contents of main saponins reached 8.7 and 9.9% in the eluting fractions of 10 and 30% ethanol, which were 32.8 and 37.3 times higher than those in DBD crude extract; and ferulic acid and ligustilide were also enriched in the eluting fractions of 10 and 70% ethanol.
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Acknowledgments
This work was supported by the International Co-operative Project of Science and Technology from the Ministry of Science and Technology of People’s Republic of China (contract/grant number: 2007DFA40680). The authors would like to thank Dr. Shunqing Hu (Hunan Industrial University) for the English modification.
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Dong, X., Liang, Y. & Wang, B. Concentration of Components in Danggui-Buxue Decoction by Macroporous Resin Chromatography Guided with LC. Chroma 72, 725–730 (2010). https://doi.org/10.1365/s10337-010-1720-9
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DOI: https://doi.org/10.1365/s10337-010-1720-9