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UPLC Analysis of Mycophenolic Acid and Its Phenol and Acyl Glucuronide Metabolites in Human Plasma

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Abstract

A simple, rapid, and reliable UPLC method has been validated for simultaneous analysis of mycophenolic acid (MPA) and its phenol glucuronide (MPAG) and acyl glucuronide (AcMPAG) metabolites in human plasma. Separation was performed on a UPLC BEH C18 column. The mobile phase was a gradient prepared from 10 mM ammonium formate and acetonitrile; the flow rate was 0.5 mL min−1, the total run time 5.5 min, and the analytes were quantified at 254 nm. Sample preparation was by liquid–liquid extraction. The method is specific, sensitive, and linear in the concentration ranges 0.062–126 μg mL−1 for MPA, 0.080–188 μg mL−1 for MPAG, and 0.031–15.9 μg mL−1 for AcMPAG.

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Acknowledgment

We thank Sabine Hadulo of Roche Palo Alto for her help in acquiring standards of MPA, MPAG, and AcMPAG.

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Author notes

  1. Lin-Yong Xu

    Present address: Institute of Clinical Pharmacology, 110 Xiangya Road, Changsha, Hunan, People’s Republic of China

Authors and Affiliations

  1. Pharmacogenetics Research Institute, Institute of Clinical Pharmacology, Central South University, 110 Xiangya Road, Changsha, 410078, Hunan, People’s Republic of China

    Dong Guo, Liang-Fang Pang, Zhi-Rong Tan, Yang Han, Hong Yang, Gan Zhou, Yao Chen, Dong-Sheng Ouyang & Hong-Hao Zhou

  2. College of Public Health, Central South University, Changsha, 410078, Hunan, People’s Republic of China

    Lin-Yong Xu

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  1. Dong Guo
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  2. Lin-Yong Xu
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Corresponding author

Correspondence to Hong-Hao Zhou.

Additional information

D. Guo and L.-Y. Xu contributed equally to this work.

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Guo, D., Xu, LY., Pang, LF. et al. UPLC Analysis of Mycophenolic Acid and Its Phenol and Acyl Glucuronide Metabolites in Human Plasma. Chroma 72, 747–752 (2010). https://doi.org/10.1365/s10337-010-1715-6

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  • DOI: https://doi.org/10.1365/s10337-010-1715-6

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