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Chromatographia

, Volume 72, Issue 3–4, pp 275–280 | Cite as

Differentiation of Lippia gracilis Schauer Genotypes by LC Fingerprint and Chemometrics Analyses

  • Silvana V. F. Gomes
  • Alan D. C. Santos
  • Valéria R. S. Moraes
  • Lúcia R. R. Martins
  • Monalisa D. Viana
  • Arie F. Blank
  • Edenir R. Pereira-Filho
  • Quezia B. Cass
  • Paulo C. L. Nogueira
  • Péricles B. Alves
Original

Abstract

A liquid chromatographic method with photodiode-array detection has been developed for differentiation of seven genotypes of Lippia gracilis Schauer, originated from Sergipe state (named 106 to 110) and from Bahia state (201 and 202), cultivated and collected in the rural campus of the Federal University of Sergipe. For a comparative analysis of the fingerprint chromatograms, chemometric tools were applied with exploratory methods. The genotypes were differentiated and their relationship with original place was established (Sergipe and Bahia states) by principal component analysis of the fingerprint chromatograms of both leaves and stem samples.

Keywords

Liquid chromatography Photodiode array detection Chemometric analysis Fingerprinting Lippia gracilis Schauer genotypes 

Notes

Acknowledgements

This research work was financially supported by the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) (Proc.620212/2006-3). Q.B.C. and A.F.B. are grateful to CNPq for research fellowships. S.V.F.G thanks UFS for a scholarship. A.D.C.S. and M.D.V. thanks CNPq for scholarships. L.R.R.M. thanks FAPESP for a scholarship. The authors would also like to thank CNPq/RENORBIO (MCT/CNPq/MS-SCTIE-DECIT/CT-Biotecnologia/CT-Saúde no 31/2006), CAPES and FAPESP.

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Copyright information

© Vieweg+Teubner Verlag | Springer Fachmedien Wiesbaden GmbH 2010

Authors and Affiliations

  • Silvana V. F. Gomes
    • 1
  • Alan D. C. Santos
    • 1
  • Valéria R. S. Moraes
    • 1
  • Lúcia R. R. Martins
    • 2
  • Monalisa D. Viana
    • 3
  • Arie F. Blank
    • 3
  • Edenir R. Pereira-Filho
    • 4
  • Quezia B. Cass
    • 4
  • Paulo C. L. Nogueira
    • 1
  • Péricles B. Alves
    • 1
  1. 1.LABORGANICS, Departamento de QuímicaUniversidade Federal de SergipeSão CristovãoBrazil
  2. 2.Departamento de Química e BiologiaUniversidade Tecnológica Federal do ParanáCuritibaBrazil
  3. 3.Departamento de Engenharia AgronômicaUniversidade Federal de SergipeSão CristovãoBrazil
  4. 4.Departamento de QuímicaUniversidade Federal de São CarlosSão CarlosBrazil

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