Differentiation of Lippia gracilis Schauer Genotypes by LC Fingerprint and Chemometrics Analyses
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A liquid chromatographic method with photodiode-array detection has been developed for differentiation of seven genotypes of Lippia gracilis Schauer, originated from Sergipe state (named 106 to 110) and from Bahia state (201 and 202), cultivated and collected in the rural campus of the Federal University of Sergipe. For a comparative analysis of the fingerprint chromatograms, chemometric tools were applied with exploratory methods. The genotypes were differentiated and their relationship with original place was established (Sergipe and Bahia states) by principal component analysis of the fingerprint chromatograms of both leaves and stem samples.
KeywordsLiquid chromatography Photodiode array detection Chemometric analysis Fingerprinting Lippia gracilis Schauer genotypes
This research work was financially supported by the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) (Proc.620212/2006-3). Q.B.C. and A.F.B. are grateful to CNPq for research fellowships. S.V.F.G thanks UFS for a scholarship. A.D.C.S. and M.D.V. thanks CNPq for scholarships. L.R.R.M. thanks FAPESP for a scholarship. The authors would also like to thank CNPq/RENORBIO (MCT/CNPq/MS-SCTIE-DECIT/CT-Biotecnologia/CT-Saúde no 31/2006), CAPES and FAPESP.
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