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A Microchip-Based Method for Rapid Separation of Subclasses of High-Density Lipoprotein

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Abstract

The subclasses of high-density lipoprotein (HDL) labeled with NBD C6-ceramide have been rapidly separated by microfluidic chip electrophoresis and detected by use of laser-induced fluorescence. The subclasses HDL2 and HDL3 were separated in 4 min. Results showed that the HDL2/HDL3 ratio for patients with CHD was much lower than that for healthy subjects. This method could meet the demand for clinical examination of HDL subclasses.

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Acknowledgments

This work was supported by grants from the National High Technology Research and Development Program (863 Program) of China (no. 2007AA042106), the 973 Program of the Ministry of Science and Technology of China (nos 2007CB714502 and 2007CB936000), the Ministry of Health (2009ZX10004-301 and 2009ZX10004-505), the Key Subject of Jiangsu Province (XK200723), and the Science and Technology Commission of Shanghai Municipality (grant nos 0752nm021, 08110700200).

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Authors and Affiliations

  1. Laboratory Medicine Center, Affiliated Hospital of Nantong University, 20 Xi Si Road, Nantong, 226001, Jiangsu, People’s Republic of China

    Huifei Zheng, Hui Cong & Huimin Wang

  2. Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences, 865 Changning Road, Shanghai, 200050, Shanghai, People’s Republic of China

    Huifei Zheng, Qinghui Jin & Jianlong Zhao

Authors
  1. Huifei Zheng
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  2. Hui Cong
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  3. Huimin Wang
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  4. Qinghui Jin
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  5. Jianlong Zhao
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Corresponding authors

Correspondence to Huimin Wang or Qinghui Jin.

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Zheng, H., Cong, H., Wang, H. et al. A Microchip-Based Method for Rapid Separation of Subclasses of High-Density Lipoprotein. Chroma 71, 1119–1124 (2010). https://doi.org/10.1365/s10337-010-1602-1

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