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Evaluation of an HPIC Method for Determination of Nitrates in Vegetables

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Abstract

The aim of the work was to verify the reliability of an HPIC method for determination of nitrates in vegetables using statistical analysis to evaluate validation parameters according the ISO/IEC 17025:2005. Standard solutions were used for the construction of a calibration curve, which was supported by Varian Star Workstation software. All calculated validation characteristics were summarized in an Excel table with final evaluation of the method. Repeatability was characterized by the selected variation coefficient sR, calculated from the standard deviation sd and arithmetic mean from a series of measurements under repeat conditions, accuracy by the variation coefficient sR, and recovery. The repeatability of the method of 3.62% is under the set limit of 5%. The calculated uncertainty was 4.22% with a recovery of 99.2%. Determination of nitrate was linear over the whole range of the calibration graph. The LOQ was 0.60 mg L−1, which is fully acceptable within the requirements for nitrate determination in plant materials. Standard uncertainty of these methods was calculated as an expanded uncertainty according to Eurachem documents in the PC program Metro2003, version 2.30.

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Acknowledgments

The authors would like to thank Professor Attila Felinger (Department of Analytical and Environmental chemistry, University of Pécs) for his kind help and advice. This work was supported by APVV (Grant no. SK-HU-0018-08).

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Authors and Affiliations

  1. Regional Authority of Public Health, Štefánikova trieda 58, 949 01, Nitra, Slovakia

    Ondrej Hegedűs

  2. Department of Chemistry, Constantine the Philosopher University in Nitra, Tr. A. Hlinku 1, 949 74, Nitra, Slovakia

    Alžbeta Hegedűsová, Silvia Jakabová & Andrea Vargová

  3. Department of Analytical and Environmental Chemistry, University of Pécs, Ifjúság útja 6, Pécs, 7624, Hungary

    Silvia Jakabová, Andrea Vargová, Tímea Pernyeszi & Borbála Boros

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  1. Ondrej Hegedűs
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  2. Alžbeta Hegedűsová
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  3. Silvia Jakabová
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Correspondence to Ondrej Hegedűs.

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Hegedűs, O., Hegedűsová, A., Jakabová, S. et al. Evaluation of an HPIC Method for Determination of Nitrates in Vegetables. Chroma 71 (Suppl 1), 93–97 (2010). https://doi.org/10.1365/s10337-010-1595-9

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  • DOI: https://doi.org/10.1365/s10337-010-1595-9

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