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Direct Measurement of Emission of Endogenous Ozone from Plants by GC–MS-SIM

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Abstract

According to recent observations antibodies can catalyze the formation of previously unknown oxidants, dihydrogen trioxide (H2O3) and ozone (O3), formed in the interaction between singlet oxygen (1O2) and H2O. The O3 molecules can be detected indirectly e.g. in TLC/OPLC chromatographic spots in the simple BioArena operating system using O3-eliminating molecules in the culture medium. It is currently supposed that plants can be used for the direct detection of O3 during its eventual emission from stomata. Using the fresh primary leaves of different plant species O3 could be really detected by means of GC–MS-SIM from the space above the the leaves which were prepared in a CO2-containing headspace vessel. It has been also established that the CO2 also contained O3 molecules, and therefore, we had to use a correction to the observed data. These new findings indicate that not only formaldehyde but also O3 and their related bioreactive compounds may play a crucial role in disease resistance (e.g. in the mechanism of action of antibodies), cell proliferation and differentiation.

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Authors and Affiliations

  1. B&B Analytics Ltd, Szt.Gellért tér 4, Budapest, 1111, Hungary

    József Balla

  2. Plant Protection Institute, Hungarian Academy of Sciences, Herman O. út 15, Budapest, 1022, Hungary

    Ernő Tyihák

Authors
  1. József Balla
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  2. Ernő Tyihák
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Correspondence to József Balla.

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Balla, J., Tyihák, E. Direct Measurement of Emission of Endogenous Ozone from Plants by GC–MS-SIM. Chroma 71 (Suppl 1), 87–91 (2010). https://doi.org/10.1365/s10337-010-1594-x

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  • DOI: https://doi.org/10.1365/s10337-010-1594-x

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