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Determination of Folic Acid by CE in Various Cultivated Variety of Lentils

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Abstract

A simple extraction and determination method for folic acid (FA) in lentil samples was developed employing capillary electrophoresis. The analysis was performed in a 75 μm ID fused silica capillary using a running buffer of 10 mM sodium borate (10%, v/v, methanol, pH 9) at +18 kV and a detection wavelength at 200 nm. Methylparaben was used as the internal standard. FA signal response was linear in the range between 1.2 × 10−5 and 4.8 × 10−5 M. Limit of detection (inter-day) was determined at 6.12 × 10−7 M (3.3 σs−1). The amount of FA found in green, red and mignon lentils was found to range between 0.408 and 0.742 mg g−1.

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Acknowledgments

The authors are grateful to the Anadolu University Scientific Research Projects (Project No 041021) for financial support and also acknowledge Onur Koyuncu for the identification of the lentils investigated in this study.

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Authors and Affiliations

  1. Department of Chemistry, Faculty of Science, Anadolu University, 26470, Eskisehir, Turkey

    Ulku Dilek Uysal & Elif Mine Oncu-Kaya

  2. Department of Analytical Chemistry, Faculty of Pharmacy, Anadolu University, 26470, Eskisehir, Turkey

    Muzaffer Tunçel

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  1. Ulku Dilek Uysal
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  2. Elif Mine Oncu-Kaya
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  3. Muzaffer Tunçel
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Correspondence to Muzaffer Tunçel.

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Uysal, U.D., Oncu-Kaya, E.M. & Tunçel, M. Determination of Folic Acid by CE in Various Cultivated Variety of Lentils. Chroma 71, 653–658 (2010). https://doi.org/10.1365/s10337-010-1555-4

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  • DOI: https://doi.org/10.1365/s10337-010-1555-4

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