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Four-Dimensional TLC on Plates with Open and Closed Adsorbent Layers

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Abstract

Four-stage TLC, a new version of multidimensional (multi-stage) planar chromatography is introduced. In the first stage a multicomponent mixture is fractionated into three groups of chromatographic zones (fractions) by TLC where the adsorbent is deposited on an aluminum foil. At the next stage, the TLC plate is dried and cut by scissors into three relatively narrow strips containing the fractions separated. At the next stages each of these strips (“daughter” plates) containing the isolated group (fractions) is separated into individual components by an appropriate mobile phase using a solvent flow perpendicular to the flow of the mobile phase in the first stage. An example of the separation of a mixture of dyes illustrates the advantages of the method in practice. This method can be expanded for separation of mixtures, containing four or more fractions. This four-dimensional version of multidimensional TLC was carried out on plates with both open (conventional) and closed adsorbent layer.

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References

  1. Mondello L, Lewis AC, Bartle KD (eds) (2002) Multidimensional Planar Chromatography, Wiley, Chichester

  2. Nyiredy Sz (2001) Multidimensional planar chromatography. In: Nyiredy Sz (ed) Planar chromatography. Springer, Budapest, pp 103–119

    Google Scholar 

  3. Nyiredy Sz (2002) Multidimensional planar chromatography. In: Mondello L, Lewis AC, Bartle KD (eds) Multidimensional Planar Chromatography. Wiley, Chichester, pp 171–196

    Google Scholar 

  4. Tuzimski T (2008) J Planar Chromatogr Mod TLC 21:49–54

    Article  CAS  Google Scholar 

  5. Geiss F (1987) In: Fundamentals of Thin Layer Chromatography (Planar Chromatography). Heidelberg, Huethig

  6. Poole CF (2003) The essence of Chromatography, Elsiver, Amsterdam

  7. Sherma J, Fried B (eds) (2003) Handbook of Thin-Layer Chromatography, Marcel Dekker, New York

  8. Reich E, Schibli A (2006) High-Perfomance Thin-Layer Chromatography for the Analysis of Medicinal Plants, Thieme, New York

  9. Sumina EG, Shtykov SN, Tyurina NV (2002) Thin-Layer Chromatography. The Theoretical Basis and Practical Application. Saratov University, Saratov

  10. Berezkin VG, Kormishkina EV (2006) J Planar Chromatogr Mod TLC 19:81–85

    Article  CAS  Google Scholar 

  11. Berezkin VG, Kulakova NY, Khrebtova SS (2009) J Planar Chromatogr Mod TLC 5:313–319

    Article  Google Scholar 

  12. Berezkin VG, Buzaev VV (1997) J Chromatogr A 758:125–134

    Article  CAS  Google Scholar 

  13. Kalmanovskii VI (2007) Metrologiya dlya khimikov (Metrology for Chemists), Yu A Nikolaev (Publ. House), Nizhnii Novgorod

Download references

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Authors and Affiliations

  1. Topchiev Institute of Petrochemical Synthesis, Russian Academy of Sciences, Leninsky pr. 29, Moscow, 119991, Russia

    Victor Berezkin, Svetlana Khrebtova & Natalia Kulakova

Authors
  1. Victor Berezkin
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  2. Svetlana Khrebtova
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  3. Natalia Kulakova
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Corresponding author

Correspondence to Victor Berezkin.

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Berezkin, V., Khrebtova, S. & Kulakova, N. Four-Dimensional TLC on Plates with Open and Closed Adsorbent Layers. Chroma 71, 907–911 (2010). https://doi.org/10.1365/s10337-010-1554-5

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  • DOI: https://doi.org/10.1365/s10337-010-1554-5

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