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Determination of Pirfenidone in Rat Plasma by LC–MS-MS and Its Application to a Pharmacokinetic Study

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Abstract

A simple liquid chromatography-electrospray ionization-mass spectrometric (LC–ESI–MS-MS) method was developed for determination of pirfenidone in rat plasma. After addition of phenacetin as internal standard, protein precipitation with 10% perchloric acid was employed for the sample preparation. Chromatographic separation was achieved on a C18 column, with 40: 60 (v/v) acetonitrile-0.1% formic acid as mobile phase. The assay is linear over the range 2–2,000 ng mL−1, with a lower limit of quantitation of 2 ng mL−1. The developed method was successfully applied to pharmacokinetic studies of pirfenidone in rats following single oral administration dose of 15 mg kg−1.

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Authors and Affiliations

  1. School of Pharmacy of Wenzhou Medical College, 325035, Wenzhou, China

    Shuhua Tong, Haiyan Jiang, Xuegu Xu, Yibin Pan, Kunming Chen, Jiao Wu, Jie Deng & Guoxin Hu

  2. Analytical and Testing Center of Wenzhou Medical College, 325035, Wenzhou, China

    Xianqin Wang

  3. The First Affiliated Hospital of Wenzhou Medical College, 325000, Wenzhou, China

    Qiaoqiao Xu

Authors
  1. Shuhua Tong
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  2. Xianqin Wang
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  3. Haiyan Jiang
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  4. Xuegu Xu
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  5. Yibin Pan
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  6. Kunming Chen
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  7. Jiao Wu
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  8. Qiaoqiao Xu
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  9. Jie Deng
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  10. Guoxin Hu
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Corresponding author

Correspondence to Guoxin Hu.

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Tong, S., Wang, X., Jiang, H. et al. Determination of Pirfenidone in Rat Plasma by LC–MS-MS and Its Application to a Pharmacokinetic Study. Chroma 71, 709–713 (2010). https://doi.org/10.1365/s10337-010-1538-5

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  • DOI: https://doi.org/10.1365/s10337-010-1538-5

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