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Determination of 18β-Glycyrrhetinic Acid in Human Urine After Ingestion of Glycyrrhizin

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Abstract

In this study, a fast and simple analytical method for determination of urinary 18β-glycyrrhetinic acid (18-GA), without any sample purification prior to the analysis, was developed. The method was successfully applied to determine the 18-GA concentration in real urine samples obtained after ingestion of glycyrrhizin. The method was shown to be specific, accurate, linear over the tested range (correlation coefficients 0.999), and repeatable (RSD below 0.2 and 3.0% for retention time and peak area, respectively). A new multi-variable-at-a-time (MVAT) approach for robustness testing gives RSD values of 0.6 and 3.8% at “−1” level, and 4.7 and 12.3% at “+1” level compared to the optimal “zero” level for retention time and peak area, respectively.

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References

  1. Bruneton J (Ed.), Pharmacognosy, Phytochemistry, Medicinal Plants. Lavoisier Publishing, Paris, France, 1999, p. 688

  2. Revers FE (1956) Acta Med Scand 312:749–751. doi:10.1111/j.0954-6820.1956.tb17084.x

    CAS  Google Scholar 

  3. Herold A, Cremer L, Călugaru A, Tamaş V, Ionescu F, Manea S, Szegli G (2003) Roum Arch Microbiol Immunol 62:217–227

    Google Scholar 

  4. Francischetti IM, Monteiro RQ, Guimaraes JA (1997) Biochem Biophys Res Commun 235:259–263. doi:10.1006/bbrc.1997.6735

    Article  CAS  Google Scholar 

  5. Mendes-Silva W, Assafim M, Ruta B, Monteiro RQ, Guimarães JA, Zingali RB (2003) Thromb Res 112:93–98. doi:10.1016/j.thromres.2003.10.014

    Article  Google Scholar 

  6. Belinky PA, Aviram M, Mahmood S, Vaya J (1998) Free Radical Biol Med 24:1419–1429. doi:10.1016/S0891-5849(98)00006-9

    Article  CAS  Google Scholar 

  7. Dhingra D, Parle M, Kulkarni SK (2004) J Ethnopharmacol 91:361–365. doi:10.1016/j.jep.2004.01.016

    Article  Google Scholar 

  8. Badam L (1997) J Commun Dis 29:91–99

    CAS  Google Scholar 

  9. Fukai T, Marumo A, Kaitou K, Kanda T, Terada S, Nomura T (2002) Life Sci 71:1449–1463. doi:10.1016/S0024-3205(02)01864-7

    Article  CAS  Google Scholar 

  10. Fukai T, Marumo A, Kaitou K, Kanda T, Terada S, Nomura T (2002) Fitoterapia 73:536–539. doi:10.1016/S0367-326X(02)00168-5

    Article  CAS  Google Scholar 

  11. Jung GD, Yang JY, Song ES, Par JW (2001) Exp Mol Med 33:131–135. doi:10.1111/j.1745-7254.2006.00435.x

    CAS  Google Scholar 

  12. Isbrucker RA, Burdock GA (2006) Regul Toxicol Pharmacol 46:167–192. doi:10.1016/j.yrtph.2006.06.002

    Article  CAS  Google Scholar 

  13. Opinion of The Scientific Committee on Food on Glycyrrhizinic Acid and its Ammonium Salt, European Commission (2003) http://ec.europa.eu/food/fs/sc/scf/out186_en.pdf (accessed April 12, 2009)

  14. Direct Food Substances Affirmed as Generally Recognized as Safe: Licorice and Licorice Derivatives (Sec. 184.1408), The Code of Federal Regulations, Title 21, Vol. 3, http://www.cfsan.fda.gov/~lrd/FCF184.html (accessed April 12, 2009).

  15. Størmer FC, Reistad R, Alexander J (1993) Food Chem Toxicol 31:303–312. doi:10.1016/0278-6915(93)90080-I

    Article  Google Scholar 

  16. Kato H, Kanaoka M, Yano S, Kobayashi M (1995) J Clin Endocrinol Metab 80:1929–1933. doi:10.1210/jc.80.6.1929

    Article  CAS  Google Scholar 

  17. Kerstens MN, Guillaume CPF, Wolthers BG, Dullaart RPF (1999) J Intern Med 246:539–547. doi:10.1046/j.1365-2796.1999.00551.x

    Article  CAS  Google Scholar 

  18. Ohtake N, Kido A, Kubota K, Tsuchiya N, Morita T, Kase Y, Takeda S (2007) Life Sci 80:1545–1552. doi:10.1016/j.lfs.2007.01.033

    Article  CAS  Google Scholar 

  19. Krähenbühl S, Hasler F, Krapf R (1994) Steroids 59:121–126. doi:10.1016/0039-128X(94)90088-4

    Article  Google Scholar 

  20. Guillaume CP, van der Molen JC, Kerstens MN, Dullaart RP, Wolthers BG (1999) J Chromatogr B Biomed Sci Appl 731:323–334. doi:10.1016/S0378-4347(99)00239-X

    Article  CAS  Google Scholar 

  21. Wang P, Li SF, Lee KH (1998) J Chromatogr A 811:219–224. doi:10.1016/S0021-9673(98)00279-9

    Article  CAS  Google Scholar 

  22. Yamamura Y, Kawakami J, Santa T, Kotaki H, Uchino K, Sawada Y, Iga T (1991) J Chromatogr 567:151–160. doi:10.1016/0378-4347(91)80319-8

    Article  CAS  Google Scholar 

  23. Hasler F, Krapf R, Brenneisen R, Bourquin D, Krähenbühl S (1993) J Chromatogr Biomed Appl 620:73–82. doi:10.1016/0378-4347(93)80053-7

    Article  CAS  Google Scholar 

  24. Raggi MA, Bugamelli F, Nobile L, Schiavone P, Cantelli-Forti G (1994) Boll Chim Farm 133:704–708

    CAS  Google Scholar 

  25. Blumenthal M (Ed.), The Complete German Commission E Monographs: Therapeutic Guide to Herbal Medicine. American Botanical Council, Austin, Texas, 1999.

  26. United States Pharmacopoeia (2007) 30th ed., National Formulary, 25th ed., United States Pharmacopoeial Convention, Rockville, MD, USA.

  27. ICH Guidelines (2005) Text on Validation of Analytical Procedures Q2(R1) 1−13, 1−13, http://www.ich.org (accessed April 12, 2009)

  28. Injac R, Kocevar N, Kreft S (2007) Anal Chim Acta 594:119–127. doi:10.1016/j.aca.2007.05.003

    Article  CAS  Google Scholar 

  29. Injac R, Boskovic M, Kocevar N, Vovk T (2008) Anal Chim Acta 620:150–161. doi:10.1016/j.aca.2008.05.007

    Article  CAS  Google Scholar 

  30. Reich E, Schibli A (2008) J AOAC Int 91:13–20

    CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Pharmaceutical Biology, Faculty of Pharmacy, University of Ljubljana, Aškerčeva Cesta 7, 1000, Ljubljana, Slovenia

    Nina Kočevar Glavač, Rade Injac & Samo Kreft

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  1. Nina Kočevar Glavač
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  2. Rade Injac
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  3. Samo Kreft
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Correspondence to Rade Injac.

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Glavač, N.K., Injac, R. & Kreft, S. Determination of 18β-Glycyrrhetinic Acid in Human Urine After Ingestion of Glycyrrhizin. Chroma 71, 917–921 (2010). https://doi.org/10.1365/s10337-010-1526-9

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  • DOI: https://doi.org/10.1365/s10337-010-1526-9

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