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Determination of Free Fatty Acids in Tibet Folk Medicine Potentilla anserina L. Using a New Labeling Reagent by LC with Fluorescence Detection and Identification with Online Atmospheric Chemical Ionization-MS Identification

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Abstract

A sensitive method for the determination of free fatty acids using 1,2-benzo-carbazole-9-ethyl-p-toluenesulfonate (BCETS) as tagging reagent with fluorescence detection has been developed. BCETS could easily and quickly label fatty acids in the presence of the K2CO3 catalyst at 80 °C for 30 min in N,N-dimethylformamide solvent. In this study, fatty acids from the extracted Potentilla anserina L. plant sample were sensitively determined. The corresponding derivatives were separated on a reversed-phase Eclipse XDB-C8 column by LC in conjunction with gradient elution. The identification was carried out by post-column APCI-MS in positive-ion detection mode. BCETS-fatty acid derivatives gave an intense molecular ion peak at m/z [M+H]+, the collision-induced dissociation spectra of m/z [M+H]+ produced the specific fragment ions at m/z [M′+CH2CH2]+, m/z 216.6 and m/z [MH−H2O]+ (here, M′: corresponding molecular mass of the fatty acids). The fluorescence excitation and emission wavelengths of the derivatives were at λ ex 279 nm and λ em 380 nm, respectively. Linear correlation coefficients for all fatty acid derivatives are more than 0.9994. Detection limits, at a signal-to-noise ratio of 3:1, are 10.79–34.19 fmol for the labeled fatty acids.

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Acknowledgments

This work was supported by the National Science Foundation of China (No. 20075016) and supported by 100 Talents Programme of The Chinese Academy of Sciences (No. 328).

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Authors and Affiliations

  1. Key Laboratory of Life-Organic Analysis of Shan Dong Province, College of Chemistry Science, Qufu Normal University, Qufu, 273165, China

    Lian Xia, Cuihua Song & Jinmao You

  2. Northwest Plateau Institute of Biology, The Chinese Academy of Sciences, Xining, 810001, China

    Lian Xia, Zhiwei Sun, Guoliang Li, Yourui Suo & Jinmao You

  3. Graduate University of Chinese Academy of Sciences, Beijing, 100049, People’s Republic of China

    Lian Xia, Zhiwei Sun & Guoliang Li

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  1. Lian Xia
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  2. Cuihua Song
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  3. Zhiwei Sun
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Correspondence to Jinmao You.

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Xia, L., Song, C., Sun, Z. et al. Determination of Free Fatty Acids in Tibet Folk Medicine Potentilla anserina L. Using a New Labeling Reagent by LC with Fluorescence Detection and Identification with Online Atmospheric Chemical Ionization-MS Identification. Chroma 71, 623–631 (2010). https://doi.org/10.1365/s10337-010-1523-z

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