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Separation and Identification of Carotenoids in Flowers of Chelidonium majus L. and Inflorescences of Solidago canadensis L.

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Abstract

The current research project aimed at the carotenoid analysis of total extracts of the inflorescences of Canadian goldenrod (Solidago canadensis L.), and the flowers of greater celandine (Chelidonium majus L.). The plant extracts were separated and analyzed by column liquid chromatography (CLC) and LC. Carotenoids were identified on the basis of their UV–Vis spectroscopic properties in different solvents, chemical reactions [5,6-epoxide → 5,8-epoxide (furanoid oxide rearrangement), (E/Z)-isomerization] and by co-chromatography with authentic reference samples. The parallel use of CLC and LC allowed the identification of numerous minor carotenoids in all extracts and fractions.

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Acknowledgments

This study was supported by the grants OTKA K 60121 and K 76176 (Hungarian National Research Foundation). We thank Mrs. A. Bognár, Mrs. J. Kriszt and Mr. N. Götz for their assistance, and Ms. Dr. N. Papp for collecting the plant materials.

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Correspondence to Györgyi Horváth.

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Horváth, G., Molnár, P., Farkas, Á. et al. Separation and Identification of Carotenoids in Flowers of Chelidonium majus L. and Inflorescences of Solidago canadensis L.. Chroma 71 (Suppl 1), 103–108 (2010). https://doi.org/10.1365/s10337-010-1510-4

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