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Simultaneous Determination of Free and Esterified Fatty Alcohols, Phytosterols and Solanesol in Tobacco Leaves by GC

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Abstract

A convenient gas chromatographic method equipped with flame ionization detection has been achieved for simultaneously determining six free and esterified fatty alcohols, three phytosterols, and solanesol in tobacco leaves. This analytic method consisted of direct alkaline hydrolysis, ultrasonic-assisted extraction and derivatization to trimethylsilyl ether derivatives. These three procedures did not require complicated and laborious preliminary purification steps of samples prior to GC, and the method was simple and convenient. The recovery of all target compounds was in the range of 85–105%, the relative standard deviations (RSDs) were less than 4%, and limits of detection (LOD) varied from 0.1–0.4 μg mL−1. The optimized methodology was successfully applied to determine free and esterified fatty alcohols, phytosterols and solanesol contents of different type tobacco samples.

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Acknowledgments

The author gratefully acknowledges the support of the technology center of China Tobacco Anhui Industrial Corporation.

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Authors and Affiliations

  1. Research Center of Tobacco and Health, University of Science and Technology of China, Hefei, 230052, China

    Yuxin Liu, Hongwu Tong & Shaomin Liu

  2. Department of Chemistry, University of Science and Technology of China, Hefei, 230026, China

    Yuxin Liu & Guoping Yong

  3. Technology Center of China Tobacco Anhui Industrial Corporation, Hefei, 230088, China

    Yingbo Xu & Dongliang Zhu

Authors
  1. Yuxin Liu
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  2. Guoping Yong
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  3. Yingbo Xu
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  4. Dongliang Zhu
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  5. Hongwu Tong
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  6. Shaomin Liu
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Corresponding author

Correspondence to Shaomin Liu.

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Liu, Y., Yong, G., Xu, Y. et al. Simultaneous Determination of Free and Esterified Fatty Alcohols, Phytosterols and Solanesol in Tobacco Leaves by GC. Chroma 71, 727–732 (2010). https://doi.org/10.1365/s10337-010-1507-z

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  • DOI: https://doi.org/10.1365/s10337-010-1507-z

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