Skip to main content
SpringerLink
Log in

LC–MS Qualitative Analysis and Simultaneous Determination of Six Filipendula Salicylates with Two Standards

  • Original
  • Published:
Chromatographia Aims and scope Submit manuscript

Abstract

Salicylate containing herbs, such as Salix alba (white willow) and Filipendula ulmaria (meadowsweet) are commonly used, because of their antipyretic, analgesic and antirheumatic effect. In this work six salicylates were identified and/or structurally characterized in the methanolic extract of F. ulmaria and were simultaneously determined using salicylic acid and salicin as external standards. Analysis occured by an LC-DAD-MS–MS triple quadrupole system, determination was accomplished in SIM mode. Linearity, inter- and intra-day precision (highest RSD % 9.85), accuracy and recovery were all within acceptable ranges, except recovery for salicin standard (51.76%). LLOQ for salicylic acid was 150 ng mL−1, for salicin 24 ng mL−1. Estimative contents of salicylates were calculated with both standard calibrations, results and their error were compared. This is the first study to provide detailed quantitative data on Filipendula salicylates.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  1. Hänsel R, Keller K, Rimpler H, Schneider G (1998) In: Hagers Handbuch der Pharmazeutischen Praxis. Folgewerk, Springer, Heidelberg, pp 147–156

  2. Zeylstra H (1998) Br J Phytother 5:8–12

    Google Scholar 

  3. Cataniciu Hintz I, Hodisan V, Tamas M (1983) Clujul Med 56:381–384

    Google Scholar 

  4. Tunon H, Olavsdotter C, Bohlin L (1995) J Ethnopharmacol 48:61–76

    Article  CAS  Google Scholar 

  5. Halkes SB, Beukelman CJ, Kroes BH, Berg AJ, Van Dijk H, Labadie RP (1997) Pharm Pharmacol Lett 7:79–82

    CAS  Google Scholar 

  6. Papp I, Simándi B, Blazics B, Alberti Á, Héthelyi É, Szőke É, Kéry Á (2008) Chromatographia 68:S125–S129. doi:10.1365/s10337-008-0749-5

    Article  CAS  Google Scholar 

  7. Güvenc A, Arihan O, Altun ML, Dinc E, Baleanu D (2007) Revista de Chimie 58:8–12

    Google Scholar 

  8. Kammerer B, Kahlich R, Biegert C, Gleiter CH, Heide L (2005) Phytochem Anal 16:470–478. doi:10.1007/s00216-005-0113-7

    Article  CAS  Google Scholar 

  9. Meier B, Lehmann D, Sticher O, Bettschart A (1987) Deutsche Apotheker Zeitung 127:2401–2407

    CAS  Google Scholar 

  10. Pemp E, Reznicek G, Krenn L (2007) J Anal Chem 62:669–673. doi:10.1134/S1061934807070106

    Article  CAS  Google Scholar 

  11. Fecka I (2009) Phytochem Anal 20:177–190. doi:10.1002/pca.1113

    Article  CAS  Google Scholar 

  12. Krasnov EA, Raldugin VA, Shilova IV, Avdeeva EY (2006) Chem Nat Comp 42:148–151. doi:10.1007/s10600-006-0064-z

    Article  CAS  Google Scholar 

  13. Papp I, Apáti P, Andrasek V, Blázovics A, Balázs A, Kursinszki L, Kite GC, Houghton PJ, Kéry Á (2004) Chromatographia 60:S93–S100

    Article  CAS  Google Scholar 

  14. Poblocka-Olech L, Van Nederkassel AM, Vander Heyden Y, Krauze-Baranowska M, Glód D, Baczek T (2007) J Sep Sci 30:2958–2966. doi:10.1002/jssc.200700137

    Article  CAS  Google Scholar 

  15. Pizzolatti MG, Mendes BG, Soldi C, Bortoluzzi JH, Carasek E (2009) J Essent Oil Res 21:255–258

    CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Pharmacognosy, Semmelweis University, Üllői u. 26, 1085, Budapest, Hungary

    Balázs Blazics, Ildikó Papp & Ágnes Kéry

Authors
  1. Balázs Blazics
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Ildikó Papp
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Ágnes Kéry
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Balázs Blazics.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Blazics, B., Papp, I. & Kéry, Á. LC–MS Qualitative Analysis and Simultaneous Determination of Six Filipendula Salicylates with Two Standards. Chroma 71 (Suppl 1), 61–67 (2010). https://doi.org/10.1365/s10337-010-1502-4

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1365/s10337-010-1502-4

Keywords

Search

Navigation