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Quantitative Characterization of Bovine Serum Albumin, α-Lactalbumin and β-Lactoglobulin in Commercial Whey Sample by RP-LC

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Abstract

A simple, sensitive and precise reverse phase liquid chromatographic method has been developed and validated for quantification of bovine serum albumin (BSA), α-lactalbumin (α-La) and β–lactoglobulin (β-Lg) that are removed from whey waste by foam fractionation method. The data is reproducible over a wide concentration range. This optimized method allowed analysis of BSA, α-La, β-Lg in a mixture within 5 min and could be applied to the analysis of a variety of commercial and laboratory whey products within a short time.

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Acknowledgments

The authors are thankful to AICTE, New Delhi, India, for providing financial assistance to carry out this project.

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Authors and Affiliations

  1. B.C.D.A College of Pharmacy and Technology, 78 Jessore Road (S) Hridaypur, Barasat, Kolkata, 700127, India

    Goutam Mukhopadhyay

  2. Department of Pharmaceutical Technology, Jadavpur University, Kolkata, 700032, India

    Jasmina Khanam & Arunabha Nanda

  3. School of Pharmacy, The University of Findlay, 1000 North Main Street, Findlay, OH, 45840, USA

    Arindam Basu Sarkar

Authors
  1. Goutam Mukhopadhyay
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  2. Jasmina Khanam
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  3. Arunabha Nanda
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  4. Arindam Basu Sarkar
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Corresponding author

Correspondence to Goutam Mukhopadhyay.

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Mukhopadhyay, G., Khanam, J., Nanda, A. et al. Quantitative Characterization of Bovine Serum Albumin, α-Lactalbumin and β-Lactoglobulin in Commercial Whey Sample by RP-LC. Chroma 71, 699–702 (2010). https://doi.org/10.1365/s10337-010-1501-5

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  • DOI: https://doi.org/10.1365/s10337-010-1501-5

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