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Determination of Four β2-Agonists in Meat, Liver and Kidney by GC–MS with Dual Internal Standards

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Abstract

A method is presented to quantitate four β2-agonists (orciprenaline, clenbuterol, salbutamol and ractopamine) in meat, liver and kidney, by GC–MS, using dual internal standards, fenoterol and metoprolol. The procedure involves liquid–liquid extraction, followed by a solid phase clean-up step and derivatization. Mass spectral acquisition was done in selected ion monitoring mode to provide a high degree of sensitivity, achieving a limit of detection of 0.04–0.09 μg kg−1 and quantitation of 0.20–0.30 μg kg−1. The typical recoveries of analytes spiked at 10, 1 and 0.1 μg kg−1 were at 80.0–102.0, 77.6–90.7 and 52.0–87.4%, respectively.

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References

  1. Wang JP, Li XW, Zhang W, Shen JZ (2006) Chromatographia 64:613–617

    Article  CAS  Google Scholar 

  2. Strydom PE, Frylinck L, Montgomery JL, Smith MF (2009) Meat Sci 81:557–564

    Article  CAS  Google Scholar 

  3. Boyd D, O’Keeffe M, Smyth MR (1994) Analyst 119:1467–1470

    Article  CAS  Google Scholar 

  4. Moane S, Smyth MR, O’Keefe M (1996) Analyst 12:779–784

    Article  Google Scholar 

  5. Courtheyn D, Desaever C, Venic (1991) J Chromatogr 564:537–549

    Article  CAS  Google Scholar 

  6. James FL, Cathie M (1997) J Chromatogr B 696:291–297

    Article  Google Scholar 

  7. Gigosos PG, Fernández TF, Maríz OC, Sampayo CAF, Abuín CF, Sáez AC (1996) J Chromatogr B677:167–172

    Google Scholar 

  8. Zhang XZ, Gan YR, Zhao FN (2003) Anal Chim Acta 489:95–101

    Article  CAS  Google Scholar 

  9. Leyssens L, Driessen C, Jacobs A, Czech J, Raus J (1991) J Chromatogr 564:515–52710

    Article  CAS  Google Scholar 

  10. Shao B, Jia XF, Zhang J, Meng J, Wu YN, Duan HJ, Tu XM (2009) Food Chem 114:1115–1121

    Article  CAS  Google Scholar 

  11. Lee HB, Sarafin K, Peart TE (2007) J Chromatogr A 1148:158–167

    Article  CAS  Google Scholar 

  12. Thevis M, Schebalkin T, Thomas A, Schänzer W (2005) Chromatographia 62:435–439

    Article  CAS  Google Scholar 

  13. Toussaint B, Hubert Ph, Tjaden UR, van der Greef J, Crommen J (2000) J Chromatogr A 871:173–180

    Article  CAS  Google Scholar 

  14. Lemesle-Lamacha V, Taverna M, Wouessidjewe D, Duchêne D, Ferrier D (1996) J Chromatogr A 735:321–331

    Article  Google Scholar 

  15. Mälkki-Laine L, Hartikainen E (1996) J Chromatogr A 724:297–306

    Article  Google Scholar 

  16. Keskin S, Özer D, Temizer A (1998) J Pharm Biomed Anal 18:639–644

    Article  CAS  Google Scholar 

  17. Henze MK, Opfermann G, Spahn-Langguth H, Schänzer W (2001) J Chromatogr B 751:93–105

    Article  CAS  Google Scholar 

  18. He LM, Su YJ, Zhang JH (2005) J Chin Mass Spectrom Soc 26:168–174

    CAS  Google Scholar 

  19. Kramer S, Blaschke G (2001) J Chromatogr B 751:169–175

    Article  CAS  Google Scholar 

  20. Martin L, Tomas A, Yong Y, Margaretha A-E, Hongwen S (2009) J Chromatogr A 1216:49–62

    Article  Google Scholar 

  21. Damasceno L, Ventura R, Cardoso J, Segura J (2002) J Chromatogr B 780:61–71

    Article  CAS  Google Scholar 

  22. Ventura R, Damasceno L, Farré M, Cardoso J, Segura J (2000) Anal Chim Acta 418:79–92

    Article  CAS  Google Scholar 

  23. Rashid BA, Kwasowski P, Stevenson D (1999) J Pharm Biomed Anal 21:635–639

    Article  CAS  Google Scholar 

  24. Zhu J, Li B, Fang XM, Chen JH, Yang JX (2005) J Chin Mass Spectrom Soc 26:129–137

    CAS  Google Scholar 

Download references

Acknowledgments

This research is sponsored by the Youth Fund of Chengdu Health Bureau (Juqing 05018). We must give personnel thanks to Ms. Wan Xiong for her editing for our manuscript.

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Authors and Affiliations

  1. West China School of Public Health, Sichuan University, Chengdu, 610041, China

    Lian Wang & Yuan-Qian Li

  2. Chengdu Center for Disease Control and Prevention, Chengdu, 610041, China

    Lian Wang & Yuan Yang

  3. Renhe District of Panzhihua Center for Disease Control and Prevention, Sichuan, 617001, China

    Yun-Ke Zhou

Authors
  1. Lian Wang
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  2. Yuan-Qian Li
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  3. Yun-Ke Zhou
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  4. Yuan Yang
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Correspondence to Lian Wang.

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Wang, L., Li, YQ., Zhou, YK. et al. Determination of Four β2-Agonists in Meat, Liver and Kidney by GC–MS with Dual Internal Standards. Chroma 71, 737–739 (2010). https://doi.org/10.1365/s10337-010-1495-z

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  • DOI: https://doi.org/10.1365/s10337-010-1495-z

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