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Simultaneous Determination of Six Phenolic Compounds in Jujube by LC-ECD

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Abstract

A simple, sensitive and accurate liquid chromatography with electrochemical detection (LC-ECD) method for simultaneous separation and determination of six phenolic compounds, including gallic acid, protocatechuic acid, caffeic acid, p-coumaric acid, rutin and quercetin in jujube has been established. ECD is more sensitive than diode-array detection (DAD) for the detection of the six phenolic compounds. All calibration curves of the phenolic compounds showed good linearity (r ≧ 0.9995) within the test ranges. The recoveries were higher than 98%. This approach can provide a reference to determine phenolic compounds in other products.

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Acknowledgments

This work was financially supported by the Science & Technology Research Program of Shaanxi Province (2009K01-09), Industrialization Program of Shaanxi Provincial Department of Education (09JC03) and the Science Foundation of Northwest University (09NW07). The authors would like to thank the graduate student Gui-Ping Qi for her precious contribution to this work.

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Authors and Affiliations

  1. College of Food Science and Engineering, Northwest A & F University, 712100, Yangling, People’s Republic of China

    Bi-Ni Wang & Ming-Tao Fan

  2. Department of Food Science and Engineering, Northwest University, 710069, Xi’an, Shaanxi, People’s Republic of China

    Bi-Ni Wang, Wei Cao & Hui Gao

  3. Institute of Analytical Science/Shaanxi Provincial Key Lab of Electroanalytical Chemistry, Northwest University, 710069, Xi’an, Shaanxi, People’s Republic of China

    Wei Cao & Jian-Bin Zheng

Authors
  1. Bi-Ni Wang
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  2. Wei Cao
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  3. Hui Gao
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  4. Ming-Tao Fan
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  5. Jian-Bin Zheng
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Correspondence to Ming-Tao Fan or Jian-Bin Zheng.

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Wang, BN., Cao, W., Gao, H. et al. Simultaneous Determination of Six Phenolic Compounds in Jujube by LC-ECD. Chroma 71, 703–707 (2010). https://doi.org/10.1365/s10337-010-1485-1

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  • DOI: https://doi.org/10.1365/s10337-010-1485-1

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