Abstract
A novel, simple and specific normal-phase liquid chromatography (NPLC) method has been developed for simultaneous determination of the four lathyrane diterpenoids, lathyrol-5,15-diacetate-3-benzoate (1, L3), lathyrol-5,15-diacetate-3,7-dibenzoate (2, L2), Δ6,17-epoxide-5,15-diacetate-3-phenylacetate (3, L1), lathyrol-5,15-diacetate-3-nicotinate (4, L8) in the hexane extract of the seeds of Euphorbia lathyris and “ZI-JIN-DING” pastille. The method showed good linearity for the four analytes (r 2 > 0.99), the intra-day and inter-day variations (RSD) were less than 3%, and the recoveries ranged from 99.4 to 100.6%. This method was successfully used to determine the four lathyrane diterpenoids in the seeds of Euphorbia lathyris and “ZI-JIN-DING” pastille, and could be applied for their quality control.
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Acknowledgment
This research was financially supported by the National Natural Science Foundation of China (No. 30670220); the Key Project of Chinese Ministry of Education (No. 107121); Program for New Century Excellent Talents in University (NCET-06-0320); Jilin Technology R&D Programme (20060904); Science Innovation Foundation of Northeast Normal University (NENU-STB07008), Changchun, China.
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Zhu, HY., Meng, XY., Bao, YL. et al. Simultaneous Determination of Lathyrane Diterpenoids by NPLC-DAD in the Seeds of Euphorbia lathyris and “ZI-JIN-DING” Pastille. Chroma 71, 617–622 (2010). https://doi.org/10.1365/s10337-010-1482-4
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DOI: https://doi.org/10.1365/s10337-010-1482-4