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Determination of Deoxynivalenol in Medicinal Herbs and Related Products by GC–ECD and Confirmation by GC–MS

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Abstract

GC with electron capture detection has been applied for the determination of deoxynivalenol (DON) in medicinal herbs and related products. The method consists of extracting the sample with water followed by clean-up of the resulting extract with an immunoaffinity column. DON was determined as its heptofluorobuturyl ester. Positive results were further confirmed by GC–MS. Recoveries from different medicinal herbs spiked with DON at levels ranging from 100 to 5,000 μg kg−1 were from 85.5 to 97.2%, with relative standard deviations (RSDs) being less than 6.3%. The limit of detection was 2.0 μg kg−1. Out of a total of 58 samples, DON was only found at low concentrations in three samples, ranging from 17.2 to 50.5 μg kg−1. This is the first report of DON determination and contamination in medicinal herbs and related products in China.

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References

  1. Sinha RC, Savard ME (1996) Can J Plant Pathol 18:233–236

    CAS  Google Scholar 

  2. Richard JL (2007) Int J Food Microbiol 119:3–10. doi:10.1016/j.ijfoodmicro.2007.07.019

    Article  CAS  Google Scholar 

  3. Muller HM, Reimann J, Schumacher U, Schwadorf K (1997) Mycopathologia 137:185–192. doi:10.1023/A:1006805717043

    Article  CAS  Google Scholar 

  4. Fazekas B, Kis M, Hajdu ET (1996) Acta Vet Hung 44:25–37

    CAS  Google Scholar 

  5. Lu G, Li L, Xue Y (1994) Chung Hua Yu Fang I Hseuh Tsa Chih 28:27–31

    CAS  Google Scholar 

  6. Ryu JC, Yang JS, Song YS, Kwon OS, Park J, Chang IM (1996) Food Addit Contam 13:333–341. doi:10.1080/02652039609374416

    CAS  Google Scholar 

  7. Schothorst RC, van Egmond HP (2004) Toxicol Lett 153:133–143. doi:10.1016/j.toxlet.2004.04.045

    Article  CAS  Google Scholar 

  8. Forsell JH, Jensen R, Tai JH, Witt M, Lin WS, Pestka JJ (1987) Food Chem Toxicol 25:155–162

    Article  CAS  Google Scholar 

  9. Rotter BA (1996) J Toxicol Environ Health 48:1–34. doi:10.1080/009841096161447

    Article  CAS  Google Scholar 

  10. Coppock RW, Swanson SP, Gelberg HB, Koritz GD, Hoffman WE, Buck WB (1985) Am J Vet Res 46:169–174

    CAS  Google Scholar 

  11. Trucksess MW, Ready DE, Pender MK, Ligmond CA, Wood GE, Page SW (1996) J AOAC Int 79:883–888

    CAS  Google Scholar 

  12. Commission Regulation 856/2005 (2005) Off J Comm L 143:3–8

    Google Scholar 

  13. Sokolovic M, Simpraga B (2006) Food Control 17:733–740. doi:10.1016/j.foodcont.2005.05.001

    Article  CAS  Google Scholar 

  14. Krska R, Baumgartner S, Josephs R (2001) Fresenius J Anal Chem 371:285–299. doi:10.1007/s002160100992

    Article  CAS  Google Scholar 

  15. Park JJ, Chu FS (1996) J AOAC Int 79:465–471

    CAS  Google Scholar 

  16. Valle-Algarra FM, Medina A, Gimento-Adelantado JV, Llorens A, Jimenez M, Mateo R (2005) Talanta 66:194–201. doi:10.1016/j.talanta.2004.11.007

    Article  CAS  Google Scholar 

  17. Schollenberger M, Lauber U, Terry-Jara H, Suchy S, Drochner W, Muller HM (1998) J Chromatogr A 815:123–132. doi:10.1016/S0021-9673(98)00454-3

    Article  CAS  Google Scholar 

  18. Jestoi M, Ritieni A, Rizzo A (2004) J Agric Food Chem 52:1464–1469. doi:10.1021/jf035130g

    Article  CAS  Google Scholar 

  19. Melchert HU, Pabel E (2004) J Chromatogr A 1056:195–199. doi:10.1016/j.chroma.2004.08.093

    CAS  Google Scholar 

  20. Cahill LM, Kruger SC, Mcalice BT, Ramsey CS, Prioli R, Kohn B (1999) J Chromatogr A 859:23–28. doi:10.1016/S0021-9673(99)00846-8

    Article  CAS  Google Scholar 

  21. Berthiller F, Schuhmacher R, Buttinger G, Krska R (2005) J Chromatogr A 1062:209–216. doi:10.1016/j.chroma.2004.11.011

    Article  CAS  Google Scholar 

  22. Biselli S, Hummert C (2005) Food Addit Contam 22:752–760. doi:10.1080/02652030500158617

    Article  CAS  Google Scholar 

  23. Sorensen LK, Elbak TH (2005) J Chromatogr B 820:183–196. doi:10.1016/j.jchromb.2005.03.020

    Article  CAS  Google Scholar 

  24. Omurtag GZ, Beyoglu D (2007) Food Control 18:163–166. doi:10.1016/j.foodcont.2005.09.007

    Article  CAS  Google Scholar 

  25. Spanjer MC, Rensen PM, Scholten JM (2008) Food Addit Contam 25:472–489. doi:10.1080/02652030701552964

    Article  CAS  Google Scholar 

  26. Santos L, Sonia M, Sanchis V, Ramos AJ (2009) J Sci Food Agric 89:1802–1807. doi:10.1002/jsfa.3647

    Article  CAS  Google Scholar 

  27. Deng GG (2005) J Chin Med Mater 28:85

    Google Scholar 

  28. Miao ZQ, Li SD, Liu XZ, Chen YJ, Li YH, Wang Y, Guo RJ, Xia ZY, Zhang KQ (2006) Sci Agric Sin 39:1371–1378

    Google Scholar 

  29. Rizzo I, Vedoya G, Maurutto S, Haidukowski M, Varsavsky E (2004) Microbiol Res 159:113–120. doi:10.1016/j.micres.2004.01.013

    Article  CAS  Google Scholar 

  30. Thrane U, Adler A, Clasen PE, Galvano F, Langseth W, Lew H, Logrieco A, Nielsen KF, Ritieni A (2004) Int J Food Microbiol 95:257–266. doi:10.1016/j.ijfoodmicro.2003.12.005

    Article  CAS  Google Scholar 

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Acknowledgments

This work was supported by the Fundamental Scientific Research Project of China (YZ-1-20), Important New Drug Research Project of the Ministry of Science and Technology of China (2009ZX09502-025) and the Scientific Research Project of Traditional Chinese Medicine Vocation in 2008 (200807042).

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Authors and Affiliations

  1. Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing, 100193, China

    Yan-Tao Yue, Xiao-Fei Zhang, Jianyu Pan & Mei-Hua Yang

  2. School of Pharmacy, Jiangsu University, Zhenjiang, 212013, China

    Yan-Tao Yue, Xiao-Fei Zhang & Zhen Ou-Yang

  3. Key Laboratory of Marine Bio-resources Sustainable Utilization, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, 510301, China

    Jun Wu

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  1. Yan-Tao Yue
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  2. Xiao-Fei Zhang
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  4. Zhen Ou-Yang
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  5. Jun Wu
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  6. Mei-Hua Yang
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Correspondence to Mei-Hua Yang.

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Yue, YT., Zhang, XF., Pan, J. et al. Determination of Deoxynivalenol in Medicinal Herbs and Related Products by GC–ECD and Confirmation by GC–MS. Chroma 71, 533–538 (2010). https://doi.org/10.1365/s10337-010-1477-1

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