Abstract
We report a sensitive and simple method for analysis of traces of Sudan dyes in foods in which solid-phase extraction on activated silica gel for preconcentration was combined, on-line, with high-performance liquid chromatography. With a loading flow rate of 1.7 mL min−1 for sampling 50 mL Sudan I–IV at pH 6.7, enrichment factors ranging from 196 to 991 were achieved. Detection limits (S/N = 3) of Sudan I–IV were in the range 1.4–7.0 ng L−1, and the relative standard deviation for repeatability of peak areas in five replicate analyses of 0.01 μg L−1 Sudan I–IV was 2.2–4.5%. When blank food samples (chilli powder, chilli sauce, and duck eggs) were spiked with Sudan III at two levels (0.25 and 0.50 μg L−1) then analyzed by this method recovery ranged from 70.3 to 95.2%.
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Abraham M, Amin M, Zissimos A (2002) Phys Chem Chem Phys 23:5748–5752. doi:10.1039/b208259d
Moller P, Wallin H (2000) Mutat Res 462:13–30. doi:10.1016/S1383-5742(99)00090-3
Stiborová M, Martinek V, Rydlova H, Hodek P, Frei E (2002) Cancer Res 62:5678–5684
Nohynek GJ, Fautz R, Benech-Kieffer F, Toutain H (2004) Food Chem Toxicol 42:517–543. doi:10.1016/j.fct.2003.11.003
International Agency for Research on Cancer (1975) Monographs on the evaluation of carcinogenic risks to humans and their supplements, vol 8, pp 225–241. BMJ 1998;317:1346–1349
Capitán F, Capitán-Vallvey LF, Fernandez MD, De Orbe I, Avidad R (1996) Anal Chim Acta 331:141–148. doi:10.1016/0003-2670(96)00192-4
Wang S, Xu ZX, Fang GZ, Duan ZJ, Zhang Y, Chen S (2007) J Agric Food Chem 55:3869–3876. doi:10.1021/jf070261t
Francesco P, Carmelo G, Francesca I, Rita M, Umile GS, Nevio P (2005) Food Chem 93:349–353. doi:10.1016/j.foodchem.2004.11.014
Du MJ, Han XG, Zhou ZH, Wu SG (2007) Food Chem 105:883–888. doi:10.1016/j.foodchem.2006.12.039
Cornet V, Govaert Y, Moens G, Van Loco J, Degroodt JM (2006) J Agric Food Chem 54:639–644. doi:10.1021/jf0517391
Erdal E, Hayrettin Ö, Cesarettin A (2007) Food Chem 105:756–760. doi:10.1016/j.foodchem.2007.01.010
He LM, Su YJ, Fang BH, Shen XG, Zeng ZL, Liu YH (2007) Anal Chim Acta 594:139–146. doi:10.1016/j.aca.2007.05.021
Mazzetti M, Fascioli R, Mazzoncini I (2004) Food Addit Contam 21:935–941. doi:10.1080/02652030400007252
Pielesz A, Baranowska I, Rybak A, Wlochowicz A (2002) Ecotox Environ Saf 53:42–47. doi:10.1006/eesa.2002.2191
Zhang YT, Zhang ZJ, Sun YH (2006) J Chromatogr A 1129:34–40. doi:10.1016/j.chroma.2006.06.028
Zhang Y, Wu HL, Xia AL, Han QJ, Cui H, Yu RQ (2007) Talanta 72:926–931. doi:10.1016/j.talanta.2006.12.019
Calbiani F, Careri M, Elviri L, Mangia A, Pistara L, Zagnoni I (2004) J Chromatogr A 1042:123–130. doi:10.1016/j.chroma.2004.05.027
Tateo F, Bononi M (2004) J Agric Food Chem 52:655–658. doi:10.1021/jf030721s
Mejia E, Ding YS, Mora MF, Garcia CD (2007) Food Chem 102:1027–1033. doi:10.1016/j.foodchem.2006.06.038
Berzas Nevado JJ, Castaneda Penalvo G, Pinilla Calderon MJ (2002) J Chromatogr B 773:151–158. doi:10.1016/S1570-0232(02)00139-3
Fang GZ, He JX, Wang S (2006) J Chromatogr A 1127:12–17. doi:10.1016/j.chroma.2006.06.024
Iamail HM, Monsour SAA, Zaki MI (1992) Thermochim Acta 202:269–280. doi:10.1016/0040-6031(92)85171-Q
Takei T, Eriguchi E, Fuji M, Watanabe T, Chikazawa M (1998) Thermochim Acta 308:139–145. doi:10.1016/S0040-6031(97)00341-9
Arakaki LNH, Augusto Filha VLS, de Sousa KS, Aguiar FP, da Fonseca MG, Espínola JGP (2006) Thermochim Acta 440:176–180. doi:10.1016/j.tca.2005.11.004
The method for the determination of Sudan dyes in foods—high performance liquid chromatography (2005) Standardization Administration of People's Republic of China, No. GB/T 19681-2005, China
Fang GZ, Tan J, Yan XP (2005) Anal Chem 77:1734–1739. doi:10.1021/ac048570v
Han DM, Fang GZ, Yan XP (2005) J Chromatogr A 1100:131–136. doi:10.1016/j.chroma.2005.09.035
Fritz JF, King JN (1976) Anal Chem 48:570–573
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The authors are grateful for financial support from the Doctor Research Foundation of the Ministry of Education of the People’s Republic of China (Project 20060057001), the China Postdoctoral Science Foundation funded project, China (project No. 20090451342), the Department of Science and Technology of Shandong Province, China (project nos 2008GG10009026 and 2009GG10009058), and the Youth Science and Technology Innovation Fund of Shandong Agricultural University, China.
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Zhixiang, X., Shuo, W., Guozhen, F. et al. On-Line SPE Coupled with LC for Analysis of Traces of Sudan Dyes in Foods. Chroma 71, 397–403 (2010). https://doi.org/10.1365/s10337-010-1472-6
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DOI: https://doi.org/10.1365/s10337-010-1472-6