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Comparison of Two On-Line Analysis Techniques Used for the Screening of Antioxidants in EGb 761

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Abstract

In order to perform on-line screening procedures for antioxidants in the Ginkgo biloba extract (EGb761), a comparison was carried out between LC–DAD–DPPH analysis used for the determination of DPPH· bleaching and LC–DAD–CL determination applied for the detection of H2O2 and O −·2 scavenging activities. The results demonstrated that 15 antioxidants in EGb 761 could be identified by LC–DAD–CL, while only 10 antioxidants were detected under LC–DAD–DPPH conditions. Furthermore, it was found that both sensitivity and reproducibility of the LC–DAD–CL method were superior. The results indicated that the combination of the two methods might provide useful information for the prediction of antioxidants in herbal medicines.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (Grant No. 30973965) and Doctoral Foundation of Xiansheng Medicine Enterprise.

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Authors and Affiliations

  1. Department of Complex Prescription of Traditional Chinese Medicine, China Pharmaceutical University, 211198, Nanjing, China

    Xiao-Ping Ding, Tian Xu, Jin Qi, Hui Wang & Bo-Yang Yu

  2. Department of Pharmacognosy, China Pharmaceutical University, 211198, Nanjing, China

    Xin-Tang Wang

  3. Key Laboratory of Modern Chinese Medicines, China Pharmaceutical University, 210009, Nanjing, China

    Bo-Yang Yu

Authors
  1. Xiao-Ping Ding
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  2. Xin-Tang Wang
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  3. Tian Xu
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  4. Jin Qi
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  5. Hui Wang
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  6. Bo-Yang Yu
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Correspondence to Bo-Yang Yu.

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Ding, XP., Wang, XT., Xu, T. et al. Comparison of Two On-Line Analysis Techniques Used for the Screening of Antioxidants in EGb 761. Chroma 71, 493–497 (2010). https://doi.org/10.1365/s10337-010-1470-8

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  • DOI: https://doi.org/10.1365/s10337-010-1470-8

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