Abstract
Scutellarin is released from the prodrug Scu-PEG after i.v. administration to rats. To investigate changes in the amount of the prodrug in vivo, a liquid chromatographic method for simultaneous analysis of Scu-PEG and scutellarin in rat plasma was developed and validated. A C18 column was used with a binary mobile phase gradient prepared from acetonitrile and 0.3% aqueous formic acid solution. The detection wavelength was 334 nm and the column temperature 40 °C. Linearity was excellent, with R 2 no less than 0.999. Intra-assay and inter-assay precision, as RSD, were less than 6.4 and 4.7%, respectively, for scutellarin and less than 2.8 and 2.9% for Scu-PEG. The method is applicable to pharmacokinetic study the prodrug and its metabolite in rats after i.v. administration.
This is a preview of subscription content, log in via an institution to check access.
References
Chen XY, Cui L, Duan XT, Ma B, Zhong DF (2006) Drug Metab Dispos 34(8):1345–1352. doi:10.1124/dmd.106.009779
Qu J, Wang Y, Luo G (2001) J Chromatogr A 919(2):437–441. doi:10.1016/S0021-9673(01)00849-4
Liu QF, Shi Y, Wang Y, Lu J, Cong WJ, Luo GA, Wang YM (2009) Talanta 80(1):84–91. doi:10.1016/j.talanta.2009.06.031
Pan ZW, Feng TM, Shan LC, Cai BZ, Chu WF, Niu HL, Lu YJ, Yang BF (2008) Phytother Res 22(11):1428–1433. doi:10.1002/ptr.2364
Lv WL, Guo JX, Li J, Huang LS, Ping QN (2005) Int J Pharm 306(1/2):99–106. doi:10.1016/j.ijpharm.2005.09.012
He Y, Zhang XX, Pan WS (2006) Chin Pharm J 41(2):119–122
Cao F, Guo JX, Ping QN (2005) Drug Dev Ind Pharm 31:747–756. doi:10.1080/03639040500216220
Zhou QS, Jiang XH, Yu JR, Li KJ (2006) Chin Chem Lett 17(1):85–88
Ye H, Zhang C, Shen WB, Sheng LS (2006) Chin J Nat Med 4:283–286
Min T, Ye H, Zhang P, Liu J, Zhang C, Shen WB, Wang W, Shen LS (2009) J Appl Polym Sci 111:444–451. doi:10.1002/app.29047
Xiong F, Wang H, Cheng J, Zhu JB (2006) J Chromatogr B 835(1/2):114–118. doi:10.1016/j.jchromb.2006.02.041
Luan LJ, Gan FP, Wu YJ (2008) Chromatographia 68(9/10):823–828. doi:10.1365/s10337-008-0763-7
Zhang ZF, Sun WX, Luo P, Wu LP, Ye LM, Zhang H (2008) J Pharm Biomed 48(3):980–985. doi:10.1016/j.jpba.2008.06.010
Huang JM, Li N, Yu YQ, Weng WY, Huang XB (2006) J Pharm Biomed 40(2):465–471. doi:10.1016/j.jpba.2005.07.051
Gao M, Huang W, RoyChowdhury M, Liu CZ (2007) Anal Chim Acta 591(2):161–166. doi:10.1016/j.aca.2007.04.004
Hao XH, Cheng G, Sun J, Zou MJ, Jie Yu, Zhang S, Cui FD (2005) J Pharm Biomed 38:360–363. doi:10.1016/j.jpba.2005.01.004
Cao F, Guo JX, Ping QN, Liao ZG (2006) Eur J Pharm Sci 29(5):385–393. doi:10.1016/j.ejps.2006.07.007
Liu QF, Luo GA, Wang YM, Ma YH, Zhang RL (2005) J Chin Med Mater 28:913–916
Huang JM, Weng WY, Huang XB, Ji YH, Chen E (2005) Eur J Drug Metab Ph 30(3):165–170
Qandil AM, Tashtoush BM, Al-Taani BM, Al-Nabulsi SM, Al-Zogoul F (2008) Chromatographia 67(3/4):287–291. doi:10.1365/s10337-007-0482-5
Chakrabarty US, Das A, Bhaumik U, Chatterjee B, Ghosh A, Bose A, Sengupta P, Nandi U, Pal TK (2009) Chromatographia 69(9/10):853–858. doi:10.1365/s10337-009-0981-7
Acknowledgment
The authors wish to thank the Jiangsu Simcere Pharmaceutical Research Institute for financial support.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Lu, J., Liu, Q., Zhao, X. et al. Simultaneous RP-LC Analysis of the Prodrug Scu-PEG and its Metabolite Scutellarin in Rat Plasma. Chroma 71, 511–515 (2010). https://doi.org/10.1365/s10337-009-1468-2
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1365/s10337-009-1468-2