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A Simple Method for Quantification of Lidocaine and Its Metabolite Monoethylglycine Xylidide in Plasma

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Abstract

We developed a simple and fast LC method quantifying lidocaine and its metabolite monoethylglycinexylidide (MEGX) in plasma. An extraction of liquid–liquid with dichloromethane and sodium hydroxide, was done using 500 μL of plasma or serum, with a UV detection at 210 nm. The results showed linearity of r = 0.996 for lidocaine and 0.998 for MEGX. Precision and accuracy showed coefficient of variation within an acceptable range. Stability test was acceptable for a period of 90 days. Levels were assayed for two patients, in the first, 1,256.14 ng mL−1 of lidocaine and 17.79 ng mL−1 of MEGX were obtained while in the second the levels for the substances were 1,194.33 and 10.47 ng mL−1. This simple and fast method is reliable and can be recommended for the quantification of lidocaine and MEGX in plasma.

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Acknowledgment

We thank Prof. Cyril Ndidi Nwoye (M.D.) for reviewing and correcting the article.

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Authors and Affiliations

  1. Laboratorio de Farmacología, Instituto Nacional de Pediatría (INP), Avenida del Imán #1 3er piso, Col Insurgentes Cuicuilco, CP 04530, Mexico City, Mexico

    Alejandra Toledo López, Guadalupe Camacho Vieyra, Hugo Juárez Olguin & Gabriela Pérez Guille

  2. Laboratorio de Cirugía Experimental, Instituto Nacional de Pediatría (INP), Avenida del Imán #1 7 piso, Col Insurgentes Cuicuilco, CP 04530, Mexico City, Mexico

    Beatriz Pérez Guille, Fernando Villegas, José González Zamora, Rosa Soriano & Miguel Jiménez-Bravo

  3. Servicio de Urgencias, Instituto Nacional de Pediatría (INP), Avenida del Imán #1, Col Insurgentes Cuicuilco, CP 04530, Mexico City, Mexico

    Omar Flores

Authors
  1. Alejandra Toledo López
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  2. Beatriz Pérez Guille
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  3. Guadalupe Camacho Vieyra
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  4. Omar Flores
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  5. Hugo Juárez Olguin
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  6. Fernando Villegas
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  7. José González Zamora
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  8. Rosa Soriano
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  9. Miguel Jiménez-Bravo
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  10. Gabriela Pérez Guille
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Corresponding author

Correspondence to Gabriela Pérez Guille.

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López, A.T., Guille, B.P., Vieyra, G.C. et al. A Simple Method for Quantification of Lidocaine and Its Metabolite Monoethylglycine Xylidide in Plasma. Chroma 71, 545–547 (2010). https://doi.org/10.1365/s10337-009-1462-8

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  • DOI: https://doi.org/10.1365/s10337-009-1462-8

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