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Preparative Isolation and Purification of Two Phenylpropanoids from Daphne giraldii Nitsche by HSCCC

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Abstract

Preparative high-speed counter-current chromatography (HSCCC) was successfully applied to purify phenylpropanoids from the stem and root bark of Daphne giraldii Nitsche, a traditional Chinese medicine. Their structures were identified on the basis of 1H NMR and 13C NMR technology. The two-phase solvent system composed of n -hexane–ethyl acetate–methanol–water (2: 3: 0.5: 4, v/v/v/v) was selected for HSCCC. A total of 8.0 mg woonenoside XI (1) and 18.0 mg daphnetin (2) were obtained in one-step separation from 200 mg of the crude extract with purity of 96.0 and 99.1%, respectively, as determined by LC. And the major compound (2) showed antithrombotic activity in vitro.

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Acknowledgment

We thank the Bureau of Science & Technology of Dalian City (2008J23JH042).

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Authors and Affiliations

  1. School of Pharmacy, Dalian Medical University, 116044, Dalian, People’s Republic of China

    Feng-Yun Li, Xiao-Chi Ma, Yan Tian, Ge Tian, Chang-Yuan Wang & Ke Xin Liu

  2. School of Pharmacology, Guangdong Medical College, 523808, Dongguan, China

    Zhan-Ping Gou

  3. Biotechnology Application Center, Beijing Vocational College of Electronic Science and Technology, Beijing, China

    Dong-Hai Su & Xiu-Lan Xin

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  1. Feng-Yun Li
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  2. Zhan-Ping Gou
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  3. Xiao-Chi Ma
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  4. Yan Tian
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  5. Ge Tian
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Correspondence to Xiao-Chi Ma.

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Li, FY., Gou, ZP., Ma, XC. et al. Preparative Isolation and Purification of Two Phenylpropanoids from Daphne giraldii Nitsche by HSCCC. Chroma 71, 481–485 (2010). https://doi.org/10.1365/s10337-009-1457-5

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  • DOI: https://doi.org/10.1365/s10337-009-1457-5

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