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Determination of Estrone and 17β-Estradiol in Water Samples Using Dispersive Liquid–Liquid Microextraction Followed by LC

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Abstract

A new method, termed dispersive liquid–liquid microextraction (DLLME), was developed for the extraction and pre-concentration of estrone (E1) and 17β-estradiol (E2) in water samples. The samples were extracted by 0.50 mL methanol (disperser solvent) containing 25.0 μL tetrachloroethane (extraction solvent). Important factors such as the volume and type of extraction and disperser solvent, extraction time and salt effect were studied. Under optimum conditions, the enrichment factors and the limits of detection were 347 and 0.2 ng mL−1 for E1, and 203 and 0.1 ng mL−1 for E2, respectively. The linear range was 0.5–5,000 ng mL−1. Compared to other methods, DLLME–LC–VWD has advantages for E1 and E2 analysis in water: high enrichment factor, low cost, simplicity, quick and easy operation.

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Acknowledgments

This work was supported in part by funding from the major project of Science and Technology Department of Zhejiang Province (NO. 2008C03001-2) and the key project of Science and Technology Department of Wenzhou (NO.S20060023).

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Authors and Affiliations

  1. Institute of Watershed Science and Environmental Ecology, Wenzhou Medical College, 325035, Wenzhou, People’s Republic of China

    Xiwei Du, Xuedong Wang, Yanyan Li, Qiaoxiang Dong & Changjiang Huang

  2. Analytical and Testing Center, Wenzhou Medical College, 325035, Wenzhou, People’s Republic of China

    Faqing Ye

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  1. Xiwei Du
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  2. Xuedong Wang
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  3. Yanyan Li
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  4. Faqing Ye
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Correspondence to Changjiang Huang.

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Du, X., Wang, X., Li, Y. et al. Determination of Estrone and 17β-Estradiol in Water Samples Using Dispersive Liquid–Liquid Microextraction Followed by LC. Chroma 71, 405–410 (2010). https://doi.org/10.1365/s10337-009-1455-7

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