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Postulating Modes of Action of Compounds with Antimicrobial Activities through Metabolomics Analysis

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Abstract

Strains of Staphylococcus aureus and coagulase-negative Staphylococcus with different antibiotic susceptibilities were selected to be the test targets of four synthesized compounds of N-aryl-4-guanidinomethylbenzoates and N-aryl-4-guanidinobenzamides. Minimum inhibitory concentrations of the four compounds showed comparable results to 13 commercial antibiotics. Metabolomics analysis based on GC–MS indicated that the four compounds shared the similar antimicrobial mechanism with clindamycin, but K–B disc diffusion tests implied that their exact binding site might be different from clindamycin or the current macrolide resistance mechanisms had no effect on the actions of synthesized compounds. The major effects of the studied compounds on the intercellular metabolites of S. aureus were the increased intracellular d-glucose, proline, phosphate and propanoic acid concentrations. This study proved metabolomics analysis was a promising tool in antibiotic mechanism research.

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Acknowledgment

We would like to express our gratitude to the technologists in Dalian Center of Clinical Laboratory.

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Authors and Affiliations

  1. Dalian Center of Clinical Laboratory, Dalian Municipal Central Hospital, 42 Xuegong Street, 116021, Dalian, China

    Yi Liu, Jie Wen, Yunduo Wang, Yan Li & Weijia Xu

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  1. Yi Liu
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  2. Jie Wen
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  3. Yunduo Wang
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  4. Yan Li
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  5. Weijia Xu
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Correspondence to Yi Liu.

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Liu, Y., Wen, J., Wang, Y. et al. Postulating Modes of Action of Compounds with Antimicrobial Activities through Metabolomics Analysis. Chroma 71, 253–258 (2010). https://doi.org/10.1365/s10337-009-1447-7

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  • DOI: https://doi.org/10.1365/s10337-009-1447-7

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