Skip to main content
Springer Nature Link
Log in

Triterpene Acids in Plantago major: Identification, Quantification and Comparison of Different Extraction Methods

  • Full Short Communication
  • Published:
Chromatographia Aims and scope Submit manuscript

Abstract

Seeds and leaves of cultivated great plantain (Plantago major L.) were extracted with conventional Soxhlet extraction using a variety of solvents and with pilot scale supercritical fluid (CO2) extraction (SFE-CO2). Hydroxy pentacyclic triterpene acids (HPTAs), oleanolic acid and ursolic acid, were identified from the SPE purified extracts with LC-(UV)-APCI-MS and quantified with LC-UV. Dried P. major leaves contained 0.07% of oleanolic acid and 0.22% of UA. Seeds had very small amounts of HPTAs, 0.005% of OA and 0.007% of ursolic acid in the oil extracted. SFE-CO2 extraction without polar modifier was found not to be a suitable technology for the leaf extraction due to the low content of lipophilic and volatile compounds. Soxhlet extraction with diethyl ether and SFE-CO2 extraction were similar in the efficiency of extracting HPTAs from the seeds.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Subscribe and save

Springer+ Basic
$34.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or eBook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Explore related subjects

Discover the latest articles and news from researchers in related subjects, suggested using machine learning.

References

  1. Samuelsen AB (2000) J Ethnopharmacol 71:1–21. doi:10.1016/S0378-8741(00)00212-9

    Article  CAS  Google Scholar 

  2. Atta AH, Abo EL-Sooud K (2004) J Ethnopharmacol 95:235–238. doi:10.1016/j.jep.2004.07.006

    Article  CAS  Google Scholar 

  3. Chiang LC, Chiang W, Chang MY, Ng LT, Lin CC (2002) Antiviral Res 55:53–62. doi:10.1016/S0166-3542(02)00007-4

    Article  CAS  Google Scholar 

  4. Ringbom T, Segura L, Noreen Y, Perera P, Bohlin L (1998) J Nat Prod 61:1212–1215. doi:10.1021/np980088i

    Article  CAS  Google Scholar 

  5. Chiang L-C, Ng LT, Chiang W, Chang M-Y, Lin C-C (2003) Planta Med 69:600–6004. doi:10.1055/s-2003-41113

    Article  CAS  Google Scholar 

  6. Heo HJ, Cho HY, Hong B, Kim HK, Heo TR, Kim EK, Kim SK, Kim CJ, Shin DH (2002) Mol Cells 13:5–11 PMID: 11911474

  7. Both DM, Goodtzova K, Yarosh DB, Brown DA (2002) Arch Dermatol Res 293:569–575. doi:10.1007/s00403-001-0272-0

    CAS  Google Scholar 

  8. Baricevic D, Sosa S, Della Laggia S, Tubaro A, Simonovska B, Krasna A, Zupancic A (2000) J Ethnopharmacol 75:125–132. doi:10.1016/s0378-8741(00)-00396-2

    Article  Google Scholar 

  9. Takeoka G, Dao L, Teranishi R, Wong R, Flessa S, Harden L, Edwards R (2000) J Agric Food Chem 48:3437–3439. doi:10.1021/jf9908289

    Article  CAS  Google Scholar 

  10. Pérez-Camino MC, Cert C (1999) J Agric Food Chem 47:1558–1562. doi:10.1021/jf980881h

    Article  Google Scholar 

  11. Janicsák G, Veres K, Kállai M, Máthé I (2003) Chromatographia 58:295–299. doi:10.1365/s10337-003-0058-y

    Google Scholar 

  12. Razboršek MI, Vončina DB, Doleček V, Vončina E (2008) Chromatographia 67:433–440. doi:10.1365/s10337-008-0533-6

    Article  Google Scholar 

  13. Chen JH, Xia ZH, Tan RX (2003) J Pharm Biochem Anal 32:1175–1179. doi:10.1016/S0731-7085(3)00160-2

    Article  CAS  Google Scholar 

  14. Zacchigna M, Cateni F, Faudale M, Sosa S, Della Loggia R (2009) Sci Pharm 77:79–86. doi:10.3797/scipharm.0809-08

    Article  CAS  Google Scholar 

  15. Wójciak-Kosior M (2007) J Pharm Biomed Anal 45:337–340. doi:10.1016/j.jpba.2007.05.011

    Article  Google Scholar 

  16. Cieśla L, Waksmundzka-Hajnos M (2009) J Chromatogr A 1216:1035–1052. doi:10.1016/j.chroma.2008.12.057

    Article  Google Scholar 

  17. Novotny L, Abdel-Hamid ME, Hamza H, Masterova I, Grancai D (2003) J Pharm Biomed Anal 2003:961–968. doi:10.1016/s0731-7085(02)00706-9

    Article  Google Scholar 

  18. Knorr R, Hamburger M (2004) J Agric Food Chem 52:3319–3324. doi:10.1021/jf049949l

    Article  CAS  Google Scholar 

  19. Cert A, Moreda W, Pérez-Camino MC (2000) J Chromatogr A 881:131–148. doi:10.1016/s0021-9673(00)00389-7

    Article  CAS  Google Scholar 

  20. Ruiz-Gutiérrez V, Pérez-Camino MC (2000) J Chromatogr A 885:321–341. doi:10.1016/s0021-9673(00)00181-3

    Article  Google Scholar 

  21. Ahmed B, Al-Howiriny TA, Mossa JS (2006) Phytochemistry 67:956–964. doi:10.1016/j.phytochem.2006.02.019

    Article  CAS  Google Scholar 

  22. Jäger S, Laszczyk MN, Scheffler A (2008) Molecules 13:3224–3235. doi:10.3390/molecules13123224

    Article  Google Scholar 

  23. Jäger S, Trojan H, Kopp T, Laszczyk MN, Scheffler A (2009) Molecules 14:2016–2031. doi:10.3390/molecules14062016

    Article  Google Scholar 

  24. Atta AH, Nasr SM, Mouneir SM (2006) Afr J Trad CAM 3:1–9 ISSN: 0189–6016

  25. Bakker M, Baas W, Sijm D, Kollöffel C (1998) Phytochemistry 47:1489–1493. doi:10.1016/s0031-9422(97)01084-4

    Article  CAS  Google Scholar 

  26. Zhannan Y, Shiqiong L, Quancai P, Chao Z, Zhengwen Y (2009) Chromatographia 69:785–790. doi:10.1365/s10337-009-0971-9

    Article  Google Scholar 

Download references

Acknowledgments

We would like to thank Petri Määttä and Petri Kauppila for the help with pilot SFE-CO2 at Aromtech Ltd. (Tornio, Finland), Eila Järvenpää for the assistance during the LC analyses at the University of Turku and Tuija Vaattovaara for the help with additional extractions and analyses at Aromtech Ltd.

Author information

Authors and Affiliations

  1. Department of Biochemistry and Food Chemistry, University of Turku, Vatselankatu 2, 20014, Turku, Finland

    Marko Tarvainen, Jukka-Pekka Suomela, Heikki Kallio & Baoru Yang

Authors
  1. Marko Tarvainen
    View author publications

    Search author on:PubMed Google Scholar

  2. Jukka-Pekka Suomela
    View author publications

    Search author on:PubMed Google Scholar

  3. Heikki Kallio
    View author publications

    Search author on:PubMed Google Scholar

  4. Baoru Yang
    View author publications

    Search author on:PubMed Google Scholar

Corresponding author

Correspondence to Marko Tarvainen.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Tarvainen, M., Suomela, JP., Kallio, H. et al. Triterpene Acids in Plantago major: Identification, Quantification and Comparison of Different Extraction Methods. Chroma 71, 279–284 (2010). https://doi.org/10.1365/s10337-009-1439-7

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1365/s10337-009-1439-7

Keywords