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LC–MS Method for the Pharmacokinetic Evaluation of 2-Arachidonoyl Glycerol in Small Volume Plasma Samples

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Abstract

A quantitative method has been developed and validated for the determination of 2-arachidonoylglycerol (2-AG) in hairless guinea pig plasma by liquid chromatographic-electrospray ionization mass spectrometry. The analytes were extracted from the plasma samples of guinea pig by a single step liquid extraction technique using acetonitrile. The chromatographic separation was conducted on a C18 column using a gradient mobile phase consisting of methanol and water at a flow rate of 0.3 mL min−1. The analytes were quantified by positive electrospray ionization mass spectrometry with selected ion monitoring mode of m/z 401. The limit of detection for 2-AG was 0.5 ng mL−1. This method required only simple processing of the samples to prevent the isomerization of 2-AG during sampling and handling and could be applied to determine the plasma concentration profiles in hairless guinea pigs. The volume of distribution at steady state (V ss), total plasma clearance (CL) and half life (t 1/2β) of 2-AG in hairless guinea pigs were 0.21 ± 0.025 L kg−1, 9.2 ± 1.5 L h−1 kg−1, and 17.7 ± 3.8 min, respectively.

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Acknowledgments

This study was supported by the American Cancer Society (RSG-00-027-04-CDD).

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Authors and Affiliations

  1. Department of Pharmaceutical Sciences, College of Pharmacy, University of Kentucky, Lexington, KY, 40536-0082, USA

    Kalpana S. Paudel, Jing Chen & Audra L. Stinchcomb

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  1. Kalpana S. Paudel
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  2. Jing Chen
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  3. Audra L. Stinchcomb
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Correspondence to Audra L. Stinchcomb.

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Paudel, K.S., Chen, J. & Stinchcomb, A.L. LC–MS Method for the Pharmacokinetic Evaluation of 2-Arachidonoyl Glycerol in Small Volume Plasma Samples. Chroma 71, 65–70 (2010). https://doi.org/10.1365/s10337-009-1413-4

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