Skip to main content
SpringerLink
Log in

An Economical Method for Isolation of Dioscin from Dioscorea nipponica Makino by HSCCC Coupled with ELSD, and a Computer-Aided UNIFAC Mathematical Model

  • Original
  • Published:
Chromatographia Aims and scope Submit manuscript

Abstract

An economical method for isolation of dioscin from Dioscorea nipponica Makino by high-speed counter-current chromatography (HSCCC) was successfully developed by using a UNIFAC mathematical model coupled with computer-aided counter-current chromatography solvent-selection software (CCC-SSS) for separate preparation of the components of the solvent system (i.e., the stationary and mobile phases). The solvent system n-hexane–ethyl acetate–ethanol–water 2:5:2:5 (v/v) was selected to demonstrate the feasibility of the approach. A comparative study was also carried out on different methods for preparation of the solvent system, namely conventional preparation of the mobile and stationary phases together in the same vessel and the method developed for separate preparation of the phases. The results indicated that purity and recovery of dioscin were no different when solvent systems prepared by the different methods were used for HSCCC separation. Much less n-hexane, ethyl acetate, and ethanol was used when the mobile and stationary phases were prepared separately, however. This was not only environmentally sensible, but also enabled conservation of resources. Use of the UNIFAC mathematical model combined with the CCC-SSS technique for separate preparation of the components of the solvent system in HSCCC is reported and explained. It is a simple and economical means of isolating pure dioscin from Dioscorea nipponica Makino.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

References

  1. Ito Y (1981) J Chromatogr A 214:122–125. doi:10.1016/S0021-9673(00)80907-3

    Article  CAS  Google Scholar 

  2. Ito Y, Sandlin JL, Bowers WG (1982) J Chromatogr A 244:247–258. doi:10.1016/S0021-9673(00)85688-5

    Article  CAS  Google Scholar 

  3. Ito Y (1988) Counter current chromatography: theory and practice. Marcel Dekker, New York, pp 79–442

    Google Scholar 

  4. Ma Y, Ito Y, Foucault A (1992) J Chromatogr A 704:75–81. doi:10.1016/0021-9673(95)00148-G

    Article  Google Scholar 

  5. Ito Y, Bowman RL (1970) Science 167:281–283. doi:10.1126/science.167.3916.281

    Article  CAS  Google Scholar 

  6. Chen LJ, Zhang Q, Yang GL, Fan LY, Tang J, Garrard I, Ignatova SN, Fisher D, Sutherland IA (2007) J Chromatogr A 1142:115–122. doi:10.1016/j.chroma.2006.09.098

    Article  CAS  Google Scholar 

  7. Xu YW, Xu LN, Yin LH, Qi Y, Han X, Xu QW, Peng JY (2007) J Dalian Med Univ 29:445–447

    CAS  Google Scholar 

  8. Peng JY, Fan GR, Qu LP, Xin Z, Wu YT (2005) J Chromatogr A 1082:203–207. doi:10.1016/j.chroma.2005.05.091

    Article  CAS  Google Scholar 

  9. Li HB, Chen F, Zhang TY, Yang FQ, Xu GQ (2001) J Chromatogr A 905:151–155. doi:10.1016/S0021-9673(00)00987-0

    Article  CAS  Google Scholar 

  10. Seger C, Eberhart K, Sturm S, Strasser H, Stuppner H (2006) J Chromatogr A 1117:67–73. doi:10.1016/j.chroma.2006.03.055

    Article  CAS  Google Scholar 

  11. Zhi WB, Deng QY, Song JN, Gu M, Ouyang F (2005) J Chromatogr A 1070:215–219. doi:10.1016/j.chroma.2005.02.065

    Article  CAS  Google Scholar 

  12. Pérez E, Santos MJ, Minguillón C (2006) J Chromatogr A 1107:165–174. doi:10.1016/j.chroma.2005.12.061

    Article  Google Scholar 

  13. Schaufelberger DE (1991) J Chromatogr A 538:45–57. doi:10.1016/S0021-9673(01)91620-6

    Article  CAS  Google Scholar 

  14. Ito Y (2005) J Chromatogr A 1065:145–168. doi:10.1016/j.chroma.2004.12.044

    Article  CAS  Google Scholar 

  15. Garrard IJ (2005) J Liq Chromatogr Relat Technol 28:1923–1935. doi:10.1081/JLC-200063571

    Article  CAS  Google Scholar 

  16. Garrard IJ, Janaway L, Fisher D (2007) J Liq Chromatogr Relat Technol 30:151–163. doi:10.1080/10826070601077252

    Article  CAS  Google Scholar 

  17. Abrams DS, Prausnitz JM (1975) AIChE J 21:116–128. doi:10.1002/aic.690210115

    Article  CAS  Google Scholar 

  18. Fredenslund A, Jones RL, Prausnitz JM (1975) AIChE J 21:1086–1099. doi:10.1002/aic.690210607

    Article  CAS  Google Scholar 

  19. Achard C, Dussap CG, Gros JB (1994) AIChE J 40:1210–1222. doi:10.1002/aic.690400712

    Article  CAS  Google Scholar 

  20. Hansen HK, Rasmussen P, Fredenslund A, Schiller M, Gmehling J (1991) Ind Eng Chem Res 30:2352–2355. doi:10.1021/ie00058a017

    Article  CAS  Google Scholar 

  21. Fredenslund A, Gmehling J, Michelsen ML, Rasmussen P, Prausnitz JM (1977) Ind Eng Chem Process Des Dev 16:450–462. doi:10.1021/i260064a004

    Article  CAS  Google Scholar 

  22. Magnussen T, Rasmussen P, Fredenslund A (1981) Ind Eng Chem Process Des Dev 20:331–339. doi:10.1021/i200013a024

    Article  CAS  Google Scholar 

  23. Cabral PP, Dubreucq E, da Fonseca MMR, Dias SF (2005) Biochem Eng J 26:29–37. doi:10.1016/j.bej.2005.06.009

    Article  Google Scholar 

  24. Kozlowska MK, Domanska U, Lempert M, Rogalski M (2005) J Chromatogr A 1068:297–305. doi:10.1016/j.chroma.2005.01.090

    Article  CAS  Google Scholar 

  25. Chen J, Zhao MQ, Yu YM, Li ZC (2007) J Chromatogr A 1151:60–64. doi:10.1016/j.chroma.2007.01.107

    Article  CAS  Google Scholar 

  26. Luo DH (2008) Carbohydr Polym 71:544–549. doi:10.1016/j.carbpol.2007.06.023

    Article  CAS  Google Scholar 

  27. Lin SH, Wang DM, Yang DP, Yao JH, Tong Y, Chen JP (2007) Anal Chim Acta 599:98–106. doi:10.1016/j.aca.2007.07.070

    Article  CAS  Google Scholar 

  28. Yuan Y, Zhang LM, Dai YJ, Yu JGJ (2007) Food Eng 82:436–440. doi:10.1016/j.jfoodeng.2007.02.055

    Article  Google Scholar 

  29. Li K, Wang YW, Gu JK, Chen XY, Zhong DF (2005) J Chromatogr B 817:271–275. doi:10.1016/j.jchromb.2004.12.026

    Article  CAS  Google Scholar 

  30. Li K, Tang YB, Fawcett JP, Gu JK, Zhong DF (2005) Steroids 70:525–530. doi:10.1016/j.steroids.2004.11.014

    Article  CAS  Google Scholar 

  31. Takechi M, Shimada S, Tanaka Y (1991) Phytochemistry 30:3943–3944. doi:10.1016/0031-9422(91)83441-M

    Article  CAS  Google Scholar 

  32. Ikeda T, Ando J, Miyazono A, Zhu XH, Tsumagari H, Nohara T (2000) Biol Pharm Bull 23:363–364. doi:10.1248/bpb.23.363

    CAS  Google Scholar 

  33. Sautour M, Mitaine-Offer AC, Miyamoto T, Dongmo A, Lacaille-Dubois MA (2004) Planta Med 70:90–92. doi:10.1055/s-2004-815467

    Article  CAS  Google Scholar 

  34. Wang Z, Zhou JB, Ju Y, Zhang H, Liu M, Li X (2001) Biol Pharm Bull 24:159–162. doi:10.1248/bpb.24.159

    Article  CAS  Google Scholar 

  35. Cai J, Liu M, Wang Z, Ju Y (2002) Biol Pharm Bull 25:193–196. doi:10.1248/bpb.25.193

    Article  CAS  Google Scholar 

  36. Liu CZ, Zhou HY, Yan Q (2007) Anal Chim Acta 582:61–68. doi:10.1016/j.aca.2006.08.057

    Article  CAS  Google Scholar 

  37. Zhai CX, Lu Q, Chen XM, Peng Y, Chen LN, Du SH (2009) J Chromatogr A 1216:2254–2262. doi:10.1016/j.chroma.2009.01.030

    Article  CAS  Google Scholar 

  38. Du SH, Liu WY, Fu TJ, Xia BG, Xia CD (2002) Yao Xue Xue Bao 37:267–270

    CAS  Google Scholar 

  39. Yang SL, Ma YH, Liu XK (2005) Yao Xue Xue Bao 40:145–149

    CAS  Google Scholar 

  40. Tan DW, Kang LP, Lü N, Zhang J, Ma BP (2006) J Chin Med Mater 29:1176–1179

    CAS  Google Scholar 

  41. Yoon KD, Kim J (2008) J Sep Sci 31:2486–2491. doi:10.1002/jssc.200800136

    Article  CAS  Google Scholar 

  42. Ma YT, Wang YT, Tuo L, Shi F, Sun WJ (2007) J Chin Hosp Pharm 27:771–773

    CAS  Google Scholar 

  43. Tschesche R, Pandey VB (1978) Phytochemistry 17:1781–1782. doi:10.1016/S0031-9422(00)88694-X

    Article  CAS  Google Scholar 

Download references

Acknowledgments

This research was supported by the Program of Key Laboratory in Liaoning Universities (no. 2008S072).

Author information

Authors and Affiliations

  1. College of Pharmacy, Dalian Medical University, 9 Western Lvshun South Road, 116044, Dalian, China

    Lian-Hong Yin, Li-Na Xu, Xiao-Na Wang, Bi-Nan Lu, Yue-Tao Liu & Jin-Yong Peng

Authors
  1. Lian-Hong Yin
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Li-Na Xu
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Xiao-Na Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Bi-Nan Lu
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Yue-Tao Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Jin-Yong Peng
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Jin-Yong Peng.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Yin, LH., Xu, LN., Wang, XN. et al. An Economical Method for Isolation of Dioscin from Dioscorea nipponica Makino by HSCCC Coupled with ELSD, and a Computer-Aided UNIFAC Mathematical Model. Chroma 71, 15–23 (2010). https://doi.org/10.1365/s10337-009-1407-2

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1365/s10337-009-1407-2

Keywords

Search

Navigation