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Determination of Anti-Tumor Agent TM208, 4-Methyl-piperazine-1-carbodithioc Acid 3-Cyano-3,3-diphenylpropyl Ester Hydrochloride, in Rats by LC

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Abstract

A new, specific and sensitive high performance liquid chromatography analytical procedure was developed and validated for the determination of TM208 in rat primary solid organs/tissues and plasma. TM208 was extracted from the appropriate matrix using methanol followed by centrifugation at 11,255×g for 20 min and injection of a 30 μL aliquot. Separation was carried out under gradient conditions using an ODS C18 column equipped with a guard column. The mobile phase consisted of methanol and water and retention times of TM208 and plunarizine (IS) were 17.658 and 26.175 min, respectively. The analytical procedure provided acceptable precision, accuracy, recoveries and linearity. Stability studies showed that TM208 was stable in organs/tissues homogenates for three freeze–thaw cycles, at room temperature for at least 24 h 3 weeks at −20 °C. The validated method was successfully applied to the determination of TM208 in SD rats following oral administration at a dose of 250 mg kg−1.

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Acknowledgement

This work was financially supported by the National Basic Research Program of China (863 Program, 2004AA2Z3783) and the National Science Foundation of China (NSFC 20172006).

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Authors and Affiliations

  1. Department of Pharmaceutical Analysis, School of Pharmaceutical Sciences, Peking University, 100083, Beijing, People’s Republic of China

    Detao Kong, Siting Wang & Xiaomei Ling

  2. State Key Laboratory of Natural and Biomimetic Drugs, Peking University, 100083, Beijing, People’s Republic of China

    Runtao Li & Jingrong Cui

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  1. Detao Kong
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  2. Siting Wang
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  3. Xiaomei Ling
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  4. Runtao Li
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  5. Jingrong Cui
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Correspondence to Xiaomei Ling or Jingrong Cui.

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Kong, D., Wang, S., Ling, X. et al. Determination of Anti-Tumor Agent TM208, 4-Methyl-piperazine-1-carbodithioc Acid 3-Cyano-3,3-diphenylpropyl Ester Hydrochloride, in Rats by LC. Chroma 70, 1721–1725 (2009). https://doi.org/10.1365/s10337-009-1402-7

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