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A Validated LC Method for Simultaneous Determination of Three Major Components in Entada phaseoloides

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Abstract

A high performance liquid chromatography method was developed for the simultaneous determination of three major active constituents in Entada phaseoloides, namely phaseoloidin (1), entadamide A (2), entadamide A-β-d-glucopyranoside (3), respectively. The samples were separated on an Aglient Eclipse XDB-C18 column with gradient elution of acetonitrile and 0.3% phosphoric acid (v/v) at a flow rate of 1.0 mL min−1 and detected at 280 nm. The three target compounds were completely separated within 10 min. All calibration curves showed good linearity (r > 0.9999) within test ranges. The reproducibility was evaluated by intra- and inter-day assays and RSD values were less than 1.04%. The recoveries were between 97.15 and 101.95%. The method was successfully applied to the analysis of three compounds in 22 commercial samples of E. phaseoloides. The results indicated that the developed LC assay was readily utilized as a quality control method for E. phaseoloides.

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  1. School of Pharmacy, Shanghai Jiao Tong University, 200240, Shanghai, People’s Republic of China

    Yuqiong Dong, Haiming Shi, Mengyue Wang & Xiaobo Li

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  1. Yuqiong Dong
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  2. Haiming Shi
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  3. Mengyue Wang
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  4. Xiaobo Li
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Correspondence to Xiaobo Li.

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Dong, Y., Shi, H., Wang, M. et al. A Validated LC Method for Simultaneous Determination of Three Major Components in Entada phaseoloides . Chroma 71, 125–129 (2010). https://doi.org/10.1365/s10337-009-1391-6

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  • DOI: https://doi.org/10.1365/s10337-009-1391-6

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