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Detection of Agmatine and Octopamine in Rat Brain and Human Plasma by Microchip Electrophoresis

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Abstract

A microchip electrophoresis method with laser induced fluorescence detection was developed for the detection of agmatine (Agm) and octopamine (Oct). The fluorescent derivatization reagent, fluorescein isothiocyanate was used for precolumn derivatization of Agm and Oct. The sodium dodecyl sulfate (SDS) micelles was employed as pseudostationary phase for the separation of Agm and Oct with other endogenous compounds exist in biological samples. Some parameters including buffer concentration, buffer pH, SDS concentration and separation voltage were investigated in detail. Under the optimum conditions, the separation and determination of Agm and Oct was performed within 40 s. The calibration curves were linear for both Agm and Oct over the concentration range of 1.0 × 10−7 to 4.0 × 10−5 M and 1.5 × 10−7 to 4.5 × 10−5 M, respectively. The detection limits of Agm and Oct (S/N = 3) are 5.0 × 10−8 and 8.0 × 10−8 M, respectively. These values make the method very suitable for the determination of Agm and Oct in rat brain tissue and human plasma as demonstrated in this paper.

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Acknowledgments

This work was financially supported by the National Natural Science Foundation of China (NSFC, Grant No. 20665002), Science Foundation of Ministry of Education, China (No. 206113), and Science Foundation of Guangxi, China (No. 0832004).

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Authors and Affiliations

  1. College of Chemistry and Chemical Engineering, Guangxi Normal University, 541004, Guilin, China

    Ming Shi, Yong Huang, Xiangtang Li & Shulin Zhao

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  1. Ming Shi
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  2. Yong Huang
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  3. Xiangtang Li
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  4. Shulin Zhao
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Correspondence to Shulin Zhao.

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Shi, M., Huang, Y., Li, X. et al. Detection of Agmatine and Octopamine in Rat Brain and Human Plasma by Microchip Electrophoresis. Chroma 70, 1651–1657 (2009). https://doi.org/10.1365/s10337-009-1387-2

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  • DOI: https://doi.org/10.1365/s10337-009-1387-2

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