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LC–DAD–ESI-MS-MS for Characterization and Quantitative Analysis of Diterpenoids from Coleus forskohlii

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Abstract

Qualitative characterization and quantitative analysis of labdane diterpenoids from Coleus forskohlii have been achieved by liquid chromatography hyphenated with photodiode-array detection and tandem electrospray ionization mass spectrometry (LC–DAD–ESI-MSn). By use of this method, thirteen forskolin-type diterpenoids were identified in the crude extract on the basis of their fragmentation mechanisms. Fragmentation rules were deduced from nine forskolin-type standards by ESI-MS in positive-ion mode. It was found that fragmentation behavior varied with the position and number of the substituents on the skeleton; this could be used for convenient identification of this type of compound. Six marker diterpenoids were also quantified, and the quality of both the cultivated and wild plants was evaluated.

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Acknowledgment

This research project was supported by the National Natural Science Foundation of China (no. 30672064).

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Authors and Affiliations

  1. Department of Natural Medicinal Chemistry, China Pharmaceutical University, 210009, Nanjing, People’s Republic of China

    Wei-Wei Zhang, Jian-Guang Luo, Jun-Song Wang, Yuan-Yuan Lu & Ling-Yi Kong

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  1. Wei-Wei Zhang
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  2. Jian-Guang Luo
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  3. Jun-Song Wang
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  4. Yuan-Yuan Lu
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Correspondence to Ling-Yi Kong.

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Zhang, WW., Luo, JG., Wang, JS. et al. LC–DAD–ESI-MS-MS for Characterization and Quantitative Analysis of Diterpenoids from Coleus forskohlii . Chroma 70, 1635–1643 (2009). https://doi.org/10.1365/s10337-009-1370-y

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