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LC Method for Quantification of ent-11α-Hydroxy-15-oxo-kaur-16-en-19-oic Acid in Rabbit Plasma: Validation and Application to a Pharmacokinetic Study

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Abstract

ent-11α-Hydroxy-15-oxo-kaur-16-en-19-oic acid (5F), a diterpenoid isolated from the Chinese herb Pteris semipinnata L, has been suggested to show antitumor properties. A simple and sensitive LC method was developed for the determination of 5F in rabbit plasma. The method involved liquid–liquid extraction using ethyl acetate under acidic conditions using naproxen as an internal standard. Separations were performed on a reversed-phase column with a mixture of 1% (v/v) glacial acetic acid and methanol (45:55, v/v) as mobile phase and UV detection was utilized at 242 nm. The calibration plot was linear in the range 0.20–10.0 μg mL−1 (correlation coefficients r 2 > 0.998). The detection limit was 0.20 μg mL−1, mean extraction recovery was above 82%, intra-day precision of the method was less than 6.4%, and inter-day precision was better than 8.7%, respectively. The validated assay was found to be suitable for the pharmacokinetic study of 5F in rabbits.

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Acknowledgements

This research was financially supported by Innovation and Technology Commission, Hong Kong (GHP/022/06) and National Natural Science Foundation of China (39870900).

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Authors and Affiliations

  1. Guangdong Key Laboratory for Research and Development of Natural Drugs, Guangdong Medical College, 524023, Zhanjiang, People’s Republic of China

    Yingnian Lu, Kefeng Wu & Nianci Liang

  2. Department of Surgery, Prince of Wales Hospital, The Chinese University of Hong Kong, Shatin, Hong Kong, People’s Republic of China

    George G. Chen

Authors
  1. Yingnian Lu
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  2. Kefeng Wu
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  3. Nianci Liang
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  4. George G. Chen
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Correspondence to Nianci Liang.

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Lu, Y., Wu, K., Liang, N. et al. LC Method for Quantification of ent-11α-Hydroxy-15-oxo-kaur-16-en-19-oic Acid in Rabbit Plasma: Validation and Application to a Pharmacokinetic Study. Chroma 70, 1599–1603 (2009). https://doi.org/10.1365/s10337-009-1368-5

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  • DOI: https://doi.org/10.1365/s10337-009-1368-5

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