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Optimization and Validation of an LC Method for the Determination of Cefdinir in Dosage Form and Human Urine

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Abstract

A simple and reliable liquid chromatographic method has been developed and validated for the determination of cefdinir in human urine and capsule samples. A chromatographic separation was achieved on a C18 column using a mobile phase consisting of potassium dihydrogen phosphate (10 mM, pH 4.5)–acetonitrile (90:10, v/v). Quantitation was achieved with UV detection at 285 nm, based on peak area with linear calibration curve at a concentration range of 0.7–39 µg mL−1. This method was successfully applied for the establishment of an urinary excretion pattern after oral dose.

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Authors and Affiliations

  1. Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Suez Canal University, Ismailia, 41522, Egypt

    Ghada M. Hadad & Waleed M. M. Mahmoud

  2. Pharmaceutical Chemistry Department, Faculty of Pharmacy, Misr International University, Km 28 Cairo-Ismailia Road, Cairo, Egypt

    Samy Emara

Authors
  1. Ghada M. Hadad
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  2. Samy Emara
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  3. Waleed M. M. Mahmoud
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Correspondence to Ghada M. Hadad.

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Hadad, G.M., Emara, S. & Mahmoud, W.M.M. Optimization and Validation of an LC Method for the Determination of Cefdinir in Dosage Form and Human Urine. Chroma 70, 1593–1598 (2009). https://doi.org/10.1365/s10337-009-1365-8

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  • DOI: https://doi.org/10.1365/s10337-009-1365-8

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