Abstract
A new LC method has been developed and validated for the direct determination of bupropion and its main metabolite, hydroxybupropion in human plasma. Plasma samples were analyzed after a simple, one step protein precipitation with trichloroacetic acid using a C8 column and mobile phase, consisting of methanol/acetonitrile/phosphate buffer (10 mM, pH 3.0) (40:10:50, v/v/v) and 20 mM 1-heptane sulfonic acid sodium salt with carbamazepine as the internal standard. UV detection was performed at 214 and 254 nm. The method was validated over the concentration range of 60–2,400 and 150–4,700 ng mL−1 for bupropion and hydroxybupropion, respectively. The intra- and inter-day assay variability was less than 15% for the two analytes. Limit of detection values were 24.8 and 63.4 ng mL−1 for bupropion and hydroxybupropion, respectively. The method developed was applied to quantification of bupropion and hydroxybupropion in human plasma.
This is a preview of subscription content, log in via an institution to check access.
References
Loboz KK, Gross AS, Ray J, McLachlan AJ (2005) J Chromatogr B 823:115–121
Richmond R, Zwar N (2003) Drug Alcohol Rev 22:203–220
Jefferson JW, Pradko JF, Muir KT (2005) Clin Ther 27:1685–1695
Hsyu PH, Singh A, Giargiari TD, Dunn JA, Ascher JA, Johnston JA (1997) J Clin Pharmacol 37:737–743
Stewart JJ, Berkel HJ, Parish RC, Simar MR, Syed A, Bocchini JA, Wilson JT, Manno JE (2001) J Clin Pharmacol 41:770–778
Johnston JA, Ascher J, Leadbetter R, Schmith VD, Patel DK, Durcan M, Bentley B (2002) Drugs 62:11–24
Cooper TB, Suckow RF, Glassman A (1984) J Pharm Sci 73:1104–1107
Suckow RF, Smith TM, Perumal AS, Cooper TB (1986) Drug Metab Dispos 14:692–697
Borges V, Yang E, Dunn J, Henion J (2004) J Chromatogr B 804:277–287
Coles R, Kharasch ED (2007) J Chromatogr B 857:67–75
Zhang D, Yuan B, Qiao M, Li F (2003) J Pharm Biomed Anal 33:287–293
Suckow RF, Zhang MF, Cooper TB (1997) Biomed Chromatogr 11:174–179
Munro JS, Walker TA (2001) J Chromatogr A 913:275–282
Munro JS, Gormley JP, Walker TA (2001) J Liq Chromatogr Related Technol 24:327–339
Castro-Puyana M, Garcia MA, Marina ML (2008) J Chromatogr B 875:260–265
Laizure SC, DeVane CL (1985) Ther Drug Monitor 7:447–450
Yeniceli D, Dogrukol-Ak D (2009) Chromatographia. Submitted for publication
Wells DA (2003) High throughput bioanalytical sample preparation methods and automation strategies. Elsevier, UK
Shah VP, Midha KK, Findlay WA, Hill HM, Hulse DJ, McGilveray IJ, Mc Key G, Miller KJ, Patnaik RN, Powell ML, Tonelli A, Viswanathan CT, Yacobi A (2000) Pharm Res 17:1551–1557
Acknowledgments
The authors appreciate the support of the Research Council of Anadolu University for our project (Project No. 060324) and GlaxoSmithKline (Istanbul, Turkey) for the gift sample of standard BUP and HBUP. D.Y. acknowledges a scholarship for Ph.D. students from the Scientific and Technical Research Council of Turkey (TUBITAK).
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Yeniceli, D., Dogrukol-Ak, D. An LC Method for the Determination of Bupropion and Its Main Metabolite, Hydroxybupropion in Human Plasma. Chroma 70, 1703–1708 (2009). https://doi.org/10.1365/s10337-009-1361-z
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1365/s10337-009-1361-z