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SPE and LC–ESI–MS for Quantitative Analysis of Mitiglinide in Human Plasma in a Bioequivalence Study

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Abstract

Liquid chromatography–electrospray ionization mass spectrometry has been used for rapid, selective, and sensitive quantitative analysis of mitiglinide in human plasma. Sample pretreatment involved solid-phase extraction from plasma with gliclazide as internal standard. Separation was performed on a C18 column (150 × 2.0 mm) with 71:29 (v/v) acetonitrile–water (containing 0.1% formic acid and 0.2 mmol L−1 ammonium acetate) as mobile phase at a flow rate of 0.2 mL min−1. The method was validated then successfully applied to a clinical bioequivalence study of mitiglinide in 20 healthy volunteers after oral administration.

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Authors and Affiliations

  1. Institute of Clinical Pharmacology, College of Medicine, Nanchang University, 461 Bayi Road, 330006, Nanchang, Jiangxi, People’s Republic of China

    Jin Hua Wen & Yu Qing Xiong

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  1. Jin Hua Wen
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  2. Yu Qing Xiong
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Correspondence to Yu Qing Xiong.

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Wen, J.H., Xiong, Y.Q. SPE and LC–ESI–MS for Quantitative Analysis of Mitiglinide in Human Plasma in a Bioequivalence Study. Chroma 70, 1715–1719 (2009). https://doi.org/10.1365/s10337-009-1358-7

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  • DOI: https://doi.org/10.1365/s10337-009-1358-7

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