Abstract
Solid-phase extraction (SPE) and reversed-phase liquid chromatography (RP-LC) have been used for simple, sensitive simultaneous analysis of cyromazine and melamine residues in liquid milk and eggs. The conditions used for SPE and LC were investigated and optimized. A combined cation-exchange–reversed-phase cartridge was used for clean-up, and an ODS (C18) column (150 mm × 4.6 mm i.d., 5-μm particles) with 62:38 (v/v) 5 mm sodium lauryl sulfate (pH 3.4)–acetonitrile as mobile phase was used for RP-LC. Under the optimum conditions the method limit of detection (LOD) for both cyromazine and melamine was 6.2 μg kg−1 for liquid milk samples, and 11.5 μg kg−1 for egg samples. Average recovery of cyromazine and melamine from milk samples was 90.3%, RSD 4.6–5.6%, and 99.6%, RSD 3.2–4.7%, respectively. Average recovery of cyromazine and melamine from egg samples was 85.3%, RSD 1.0–4.7%, and 89.6%, RSD 3.1–5.0%, respectively. The method enables detection of melamine and cyromazine at levels as low as 20.7 μg kg−1 in liquid milk and 38.3 μg kg−1 in egg.
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This work was supported by the Nature Science Funds of Hebei Province of China (B2008000583).
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Sun, H., Wang, L., Liu, N. et al. SPE then RP-LC for Simultaneous Analysis of Cyromazine and its Metabolite Melamine in Liquid Milk and Egg. Chroma 70, 1685–1689 (2009). https://doi.org/10.1365/s10337-009-1355-x
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DOI: https://doi.org/10.1365/s10337-009-1355-x