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Quantitative Analysis of Busulfan in Human Plasma by LC-MS–MS

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Abstract

High-performance liquid chromatography with tandem mass spectrometry has been used for rapid, specific, and sensitive analysis of busulfan in human plasma. Busulfan-d8 was used as internal standard. Analysis was performed on a C18 column (50 mm × 2.1 mm, 3.5-µm particles) with water–methanol 80:20 (v/v) as mobile phase at a flow-rate of 0.30 mL min−1. Detection was by tandem triple–quadrupole mass spectrometry with turbo ion-spray ionization. Linear calibration plots were obtained over the concentration range 1.096–1,096 ng mL−1. The assay is ideally suited to monitoring of busulfan and determination of its pharmacokinetic data.

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Authors and Affiliations

  1. Hygiene Detection Center, School of Public Health and Tropical Medicine, Southern Medical University, 510515, Guangzhou, China

    Lingyun Chen, Zhifeng Zhou, Mei Shen & Ande Ma

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  1. Lingyun Chen
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  2. Zhifeng Zhou
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  3. Mei Shen
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  4. Ande Ma
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Correspondence to Ande Ma.

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Chen, L., Zhou, Z., Shen, M. et al. Quantitative Analysis of Busulfan in Human Plasma by LC-MS–MS. Chroma 70, 1727–1732 (2009). https://doi.org/10.1365/s10337-009-1352-0

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  • DOI: https://doi.org/10.1365/s10337-009-1352-0

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