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Stability-Indicating LC-PDA Method for Determination of Idebenone in Nanoparticles Based on Chitosan and N-Carboxymethylchitosan

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Abstract

A forced degradation study of idebenone was conducted under conditions of UV irradiation, acid, basic and oxidative hydrolysis and in order to develop an isocratic stability-indicating LC-UV method for drug quantification in chitosan and N-carboxymethylchitosan nanoparticles obtained by spray drying. The drug was more labile to alkaline treatment than under the other forced degradation conditions. Idebenone and its degradation products were optimally resolved (resolution >4) on a Luna Phenomenex C18 column with mobile phase composed by methanol:water: (80:20% v/v) at a flow rate of 1.0 mL min−1, at 30 °C, using wavelength of 279 nm for drug detection. The method was linear, over a drug concentration range of 2 to 10 μg mL−1. The RSD% value of intra- and inter-day precision studies was <1.5. The method showed excellent recoveries (99.4 to 101.1%). The LOD and LOQ values were found to be 0.18 and 0.59 μg mL−1, respectively. In conclusion this method can be used as a rapid and accurate assay of idebenone in the nanoparticles during stability tests.

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Acknowledgments

The authors are grateful to CNPq (Rede Brasileira de Nanoglicobiotecnologia and Edital Universal) for the financial support during this study. We thank Drs. Antonio J. Pierik and Daili J. A. Netz (Philipps University, Marburg, Germany) for critically reading the manuscript.

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Authors and Affiliations

  1. Núcleo de Investigações Químico-Farmacêuticas (Niqfar),Programa de Mestrado em Ciências Farmacêuticas, Universidade do Vale do Itajaí (UNIVALI), P. O. Box 360, Itajaí, SC, 88302-202, Brazil

    Clarissa M. de Amorim, Daisy J. A. Netz, Angelica G. Couto, Rilton A. de Freitas & Tania M. B. Bresolin

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  1. Clarissa M. de Amorim
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  2. Daisy J. A. Netz
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  3. Angelica G. Couto
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  4. Rilton A. de Freitas
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  5. Tania M. B. Bresolin
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Correspondence to Tania M. B. Bresolin.

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de Amorim, C.M., Netz, D.J.A., Couto, A.G. et al. Stability-Indicating LC-PDA Method for Determination of Idebenone in Nanoparticles Based on Chitosan and N-Carboxymethylchitosan. Chroma 70, 1411–1415 (2009). https://doi.org/10.1365/s10337-009-1350-2

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  • DOI: https://doi.org/10.1365/s10337-009-1350-2

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