Abstract
The chemometric approach combining Box–Behnken response surface model and Derringer’s desirability function was applied for simultaneous optimization of resolution and analysis time in countercurrent chromatography (CCC). The mergence of the two parameters was accomplished using the Derringer’s desirability function with subsequent optimization by a Box–Behnken response surface design. The developed model was checked by statistical analysis. By implementing the optimal flow rate, rotation speed and temperature predicted by the validated model, enhanced resolution between two similar analytes (phenol and resorcinol) was achieved in a reasonable time. The analyses and results obtained in this paper will benefit to improve the efficiency of CCC separation.
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Acknowledgments
The authors would like to acknowledge financial support from the Foundation of Key Laboratory of Bioprocess of Beijing (grant No. SYS 100100421), the National Natural Science Foundation of China (grant No. 20576010) and the Program for New Century Excellent Talents (NCET-05-0117).
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Du, X., Li, Y. & Yuan, Q. Chemometric Approach for Simultaneous Optimization of Resolution and Analysis Time in CCC. Chroma 70, 1547–1552 (2009). https://doi.org/10.1365/s10337-009-1343-1
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DOI: https://doi.org/10.1365/s10337-009-1343-1