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Screening and Identification of Permeable Components of Radix et Rhizoma Rhei Extract by Use of Immobilized Artificial Membrane Chromatography

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Abstract

The objective of this work was to screen and identify permeable components of Radix et Rhizoma Rhei extract by use of immobilized artificial membrane chromatography (IAMC) then to investigate the intestinal absorption behavior of these components and the mechanism of absorption at three concentrations (0.3, 0.6, and 1.5 mg mL−1) by use of the in situ rat intestinal absorption model. The percentage absorption of permeable components was then correlated with their retention behavior by using mobile phases of three different pH (5.0, 6.0, and 7.0) containing buffers of two different ionic strength (10 and 50 mmol L−1). There was better correlation between percentage intestinal absorption and IAMC retention at pH 6.0 (r 2 = 0.82) than at pH 5.0 (r 2 = 0.64) or 7.0 (r 2 = 0.74). The correlation coefficients suggest that most of the permeable components of Radix et Rhizoma Rhei were mainly absorbed by a passive diffusion mechanism. The four main permeable components were identified as rhein, aloe-emodin, chrysophanol, and emodin by comparison of chromatographic and spectrometric behavior with those of reference standards. IAMC seems useful as a promising rapid screening technique for identification of bioactive components in complex systems, e.g. traditional Chinese medicines.

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References

  1. Robert SA (2003) Curr Opin Drug Discov Dev 6:66–80

    Google Scholar 

  2. Sun J, Wu X, Lu R, Liu JF, Wang YJ, He ZG (2008) Curr Drug Metab 9:152–166

    Article  CAS  Google Scholar 

  3. Ong SW, Liu HL, Pidgeon C (1996) J Chromatogr A 728:113–128

    Article  CAS  Google Scholar 

  4. Taillardat-Bertschinger A, Carrupt PA, Barbato F, Testa B (2003) J Med Chem 46:655–665

    Article  CAS  Google Scholar 

  5. Ong S, Liu H, Qiu X, Bhat G, Pidgeon C (1995) Anal Chem 67:755–762

    Article  CAS  Google Scholar 

  6. Nasal A, Sznitowska M, Bucifiski A, Kaliszan R (1995) J Chromatogr A 692:83–89

    Article  CAS  Google Scholar 

  7. Pidgeon C, Ong S, Liu H, Qiu X, Pidgeon M, Dantzig AH, Munroe J, Hornback WJ, Kasher JS, Glunz L, Szczerba T (1995) J Med Chem 38:590–594

    Article  CAS  Google Scholar 

  8. Sun J, Sakai S, Tauchi Y, Deguchi Y, Cheng G, Chan J, Morimoto K (2003) Eur J Pharm Biopharm 56:223–229

    Article  CAS  Google Scholar 

  9. Kotecha J, Shah S, Rathod I, Subbaiah G (2008) Int J Pharm 360:96–106

    Article  CAS  Google Scholar 

  10. White RE (2000) Annu Rev Pharmacol Toxicol 40:133–157

    Article  CAS  Google Scholar 

  11. Wang HL, Zou HF, Kong L, Ni J (2000) J Basic Clin Physiol Pharmacol 11:155–172

    Google Scholar 

  12. Brasitus TA, Dudeja PK, Dahiya R, Halline A (1987) Biochem J 248:455–461

    CAS  Google Scholar 

  13. Beigi F, Gottschalk CL, Hagglund L, Brekkan E, Zhang YX, Osterberg T, Lundahl P (1998) Int J Pharm 164:129–137

    Article  CAS  Google Scholar 

  14. Kong L, Zou H, Wang H, Ni J, Zhang Y (2000) Chem J Chin Univ 21:36–40

    CAS  Google Scholar 

  15. Sheng LH, Li P, Zou HF (2005) Chin J Anal Chem 33:14–18

    Google Scholar 

  16. Zhou X, Song B, Jin L, Hu D, Diao C, Xu G, Zou Z, Yang S (2006) Bioorg Med Chem Lett 16:563–568

    Article  CAS  Google Scholar 

  17. Su HY, Chemg SH, Chen CC (1995) Mutat Res 329:205–212

    CAS  Google Scholar 

  18. Zhang L, Chang CJ, Bacus SS (1995) Cancer Res 55:3890–3896

    CAS  Google Scholar 

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Acknowledgment

We are grateful for financial support from the National Natural Science Foundation of China (nos. 30400563 and 30672556) and the Natural Science Foundation of Liaoning Province (nos 20052058 and 20062058).

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Authors and Affiliations

  1. Department of Biopharmaceutics, School of Pharmacy, Shenyang Pharmaceutical University, 103 Wenhua Road, 110016, Shenyang, China

    Wenping Zhang, Jin Sun, Yongjun Wang, Xiaohong Liu, Yinghua Sun, Rong Lu & Zhonggui He

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  1. Wenping Zhang
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  2. Jin Sun
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  3. Yongjun Wang
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  4. Xiaohong Liu
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  5. Yinghua Sun
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  7. Zhonggui He
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Correspondence to Jin Sun or Zhonggui He.

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Zhang, W., Sun, J., Wang, Y. et al. Screening and Identification of Permeable Components of Radix et Rhizoma Rhei Extract by Use of Immobilized Artificial Membrane Chromatography. Chroma 70, 1321–1326 (2009). https://doi.org/10.1365/s10337-009-1342-2

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