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Simultaneous Determination of Dopamine, Epinephrine and 5-Hydroxytryptamine in Toad Venom and Common Yam Rhizome by MEKC

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Abstract

A micellar electrokinetic chromatography method was developed for the simultaneous determination of dopamine, epinephrine and 5-hydroxytryptamine. Several experimental parameters such as surfactant type and concentration, buffer concentration and pH, type and concentration of organic modifier were evaluated for the analysis of the studied compounds. Among the investigated separation conditions, the composition of micelles, pH and the methanol concentration were the critical parameters. Dopamine, epinephrine and 5-hydroxytryptamine were separated and determined successfully within 7.5 min in Toad venom and Common yam rhizome under the optimum conditions.

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Acknowledgments

This work was financially supported by doctoral foundation of the Southwest University of Science and Technology (07ZX7130).

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Authors and Affiliations

  1. School of National Defense Science and Technology, Southwest University of Science and Technology, 621010, Mianyang, Sichuan, China

    Shumin Wang

  2. Department of Chemistry, Lanzhou University, 730000, Lanzhou, China

    Zhi Luo, Weiping Wang, Xingguo Chen & Zhide Hu

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  1. Shumin Wang
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  2. Zhi Luo
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  3. Weiping Wang
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Correspondence to Shumin Wang.

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Wang, S., Luo, Z., Wang, W. et al. Simultaneous Determination of Dopamine, Epinephrine and 5-Hydroxytryptamine in Toad Venom and Common Yam Rhizome by MEKC. Chroma 70, 1467–1471 (2009). https://doi.org/10.1365/s10337-009-1333-3

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