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Determination of T-2 Toxin in Traditional Chinese Herbal Medicines by GC-ECD

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Abstract

Gas chromatography with electron capture detection has been applied for the determination of T-2 toxin (T-2) in traditional Chinese herbal medicines (TCHM). The method consists of extracting the sample with aqueous methanol followed by cleanup of the resulting extract with an immunoaffinity column. T-2 was determined as its heptafluorobutyl ester. The reaction temperature and time of derivatization were investigated to obtain the optimum conditions. Recoveries from different TCHMs, spiked with T-2 at levels ranging from 50 to 1,000 μg kg−1, were from 82.2 to 98.6%, with relative standard deviations of less than 7.5%. The limit of detection was 2.5 μg kg−1. Out of 32 commercially available TCHM samples analyzed, none were found to contain any detectable amount of T-2.

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Acknowledgments

This work was supported by Fundamental Scientific Research Project of China (YZ-1-20), Important New Drug Research Project of the Ministry of Science and Technology of China (2009ZX09502-025) and Scientific Research Project of Traditional Chinese Medicine Vocation in 2008 (200807042).

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Authors and Affiliations

  1. Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College, 100193, Beijing, China

    Yan-Tao Yue, Xiao-Fei Zhang, Wei-Wei Gao & Mei-Hua Yang

  2. School of Pharmacy, Jiangsu University, 212013, Zhenjiang, China

    Yan-Tao Yue, Xiao-Fei Zhang & Zhen Ou-Yang

  3. Key Laboratory of Marine Bio-resources Sustainable Utilization, South China Sea Institute of Oceanology, Chinese Academy of Sciences, 510301, Guangzhou, China

    Jun Wu

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  1. Yan-Tao Yue
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  2. Xiao-Fei Zhang
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  3. Zhen Ou-Yang
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  5. Jun Wu
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Correspondence to Mei-Hua Yang.

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Yue, YT., Zhang, XF., Ou-Yang, Z. et al. Determination of T-2 Toxin in Traditional Chinese Herbal Medicines by GC-ECD. Chroma 70, 1495–1499 (2009). https://doi.org/10.1365/s10337-009-1330-6

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  • DOI: https://doi.org/10.1365/s10337-009-1330-6

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