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LC−UV Analysis of N-{3-(2,4-Dioxo-1,4-dihydro-2H-quinazolin-3-yl)propyl}-N-[4-{3-(2,4-dioxo-1,4-dihydro-2H-quinazolin-3-yl)propyl-amino}butyl]acetamide (SP-8203) in Rat Plasma, Urine, and Gastrointestinal Tract Samples

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Abstract

A simple, rapid, and reproducible reversed-phase LC method with UV detection at 215 nm has been developed for analysis of SP-8203 in rat samples. A C18 column was used with 3,000:1,050 (v/v) 0.01 m K2HPO4 buffer (pH 3)–acetonitrile as mobile phase at a flow rate of 1.7 mL min−1 at 50 °C. Samples were extracted with dichloromethane containing ondansetron (internal standard). Detection limits for SP-8203 in plasma, urine, and gastrointestinal tract samples were 0.05, 0.5, and 10 μg mL−1, respectively. The method was suitable for pharmacokinetic study of SP-8203 in rats after intravenous administration.

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Acknowledgments

This study was supported by the contract ‘Pharmacokinetics of SP-8203’ from Shin Poong Pharmaceutical Company, Ltd, Ansan, South Korea.

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Author notes

  1. Joo Hyun Lee

    Present address: Gastroenterology and Metabolism Products Division, Drug Evaluation Department, Pharmaceutical Safety Bureau, Korea Food and Drug Administration, Seoul, South Korea

Authors and Affiliations

  1. College of Pharmacy and Research Institute of Pharmaceutical Sciences, Seoul National University, San 56-1, Shinlim-Dong, Kwanak-Gu, Seoul, 151-742, South Korea

    Young Hee Choi, Jung Hwa Suh, Joo Hyun Lee & Myung Gull Lee

  2. Central Research Institute, Shin Poong Pharmaceutical Company, Ltd, 434-4, Moknae-Dong, Danwon-Gu, Ansan, Kyunggi-Do, 425-10, South Korea

    Il Hwan Cho & Chul Kyu Lee

Authors
  1. Young Hee Choi
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  2. Jung Hwa Suh
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  3. Joo Hyun Lee
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  4. Il Hwan Cho
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  5. Chul Kyu Lee
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  6. Myung Gull Lee
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Correspondence to Myung Gull Lee.

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Choi, Y.H., Suh, J.H., Lee, J.H. et al. LC−UV Analysis of N-{3-(2,4-Dioxo-1,4-dihydro-2H-quinazolin-3-yl)propyl}-N-[4-{3-(2,4-dioxo-1,4-dihydro-2H-quinazolin-3-yl)propyl-amino}butyl]acetamide (SP-8203) in Rat Plasma, Urine, and Gastrointestinal Tract Samples. Chroma 70, 1435–1439 (2009). https://doi.org/10.1365/s10337-009-1327-1

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  • DOI: https://doi.org/10.1365/s10337-009-1327-1

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