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Comparative LC Enantioseparation of Novel PPAR Agonists on Cellulose- and Amylose-Based Chiral Stationary Phases

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Abstract

The LC enantiomeric separation of several dual PPARα/γ agonists on the commercially available Chiralcel OD and Chiralpak AD columns has been evaluated in normal phase mode using a mobile phase consisting in a mixture of n-hexane, 2-propanol and trifluoroacetic acid at constant volume ratio. Most compounds were separated as underivatized acids without requiring time consuming analysis. Some complementary selectivity was evidenced on the two investigated chiral stationary phases related to the different accessibility of the active sites of the helical cavities. Additional information on the chiral recognition mechanism were deduced from the chromatographic behaviour of some selected methyl esters.

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Authors and Affiliations

  1. Dipartimento Farmaco-Chimico, Università degli Studi di Bari, via Orabona 4, 70126, Bari, Italy

    Luca Piemontese, Salvatore Faliti, Giuseppe Carbonara, Antonio Laghezza, Paolo Tortorella & Fulvio Loiodice

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  1. Luca Piemontese
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  2. Salvatore Faliti
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  3. Giuseppe Carbonara
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  4. Antonio Laghezza
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  5. Paolo Tortorella
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  6. Fulvio Loiodice
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Correspondence to Fulvio Loiodice.

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Piemontese, L., Faliti, S., Carbonara, G. et al. Comparative LC Enantioseparation of Novel PPAR Agonists on Cellulose- and Amylose-Based Chiral Stationary Phases. Chroma 70, 1327–1333 (2009). https://doi.org/10.1365/s10337-009-1323-5

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  • DOI: https://doi.org/10.1365/s10337-009-1323-5

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