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Amino Acid Profile Determination in the Urine of Bladder Cancer Patients by CE-MS with On-Line pH-Mediated Stacking and Pattern Recognition

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Abstract

A rapid and sensitive capillary electrophoresis-mass spectrometry with on-line pH-mediated stacking method for the simultaneous quantification of 19 amino acids in urine was established and applied for the analysis of the urinary amino acids of 20 bladder cancer patients and 20 healthy subjects. The two groups could be well classified by pattern recognition, which indicated that the two groups had different urinary amino acids profiles. Compared to those of healthy subjects, the relative concentrations of three glycogenic amino acids (methionine, cysteine and valine) decreased significantly in the urine of bladder cancer patients.

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References

  1. van Rhijn BWG, van der Poel HG, van der Kwast TH (2005) Eur Urol 47:736–748. doi:10.1016/j.eururo.2005.03.014

    Article  Google Scholar 

  2. Masuda M, Nishihira T, Itoh K, Mizugaki M, Ishida N, Mori S (1993) Cancer 72:3571–3578. doi:10.1002/1097-0142(19931215)72:12<3571:AID-CNCR2820721205>3.0.CO;2-9

    Article  CAS  Google Scholar 

  3. Vrooman OPJ, Witjes JA (2008) Eur Urol 53:909–916. doi:10.1016/j.eururo.2007.12.006

    Article  Google Scholar 

  4. Cascino A, Muscaritoli M, Cangiano C, Conversano L, Laviano A, Ariemma S, Meguid MM, Rossi Fanelli F (1995) Anticancer Res 15:507–510

    CAS  Google Scholar 

  5. Kubota A, Meguid MM, Hitch DC (1992) Cancer 69:2343–2348. doi:10.1002/1097-0142(19920501)69:9<2343:AID-CNCR2820690924>3.0.CO;2-S

    Article  CAS  Google Scholar 

  6. McGregor RF, Johnson DE, Sharon MS, Crawford R, Brown B, Johnston D (1977) Urology 9:538–542. doi:10.1016/0090-4295(77)90247-3

    Article  CAS  Google Scholar 

  7. Deyl Z, Hyanek J, Horakova M (1986) J Chromatogr 379:177–250. doi:10.1016/S0378-4347(00)80685-4

    Article  CAS  Google Scholar 

  8. Yu K, Sheng GP, Sheng JF, Chen YM, Xu W, Liu XL, Cao HC, Qu HB, Cheng YY, Li LJ (2007) J Proteome Res 6:2413–2419. doi:10.1021/pr060591d

    Article  CAS  Google Scholar 

  9. Prata C, Bonnafous P, Fraysse N, Treilhou M, Poinsot V, Couderc F (2001) Electrophoresis 22:4129–4138. doi:10.1002/1522-2683(200111)22:19<4129:AID-ELPS4129>3.0.CO;2-I

    Article  CAS  Google Scholar 

  10. Klampfl CW, Ahrer W (2001) Electrophoresis 22:1579–1584. doi:10.1002/1522-2683(200105)22:8<1579:AID-ELPS1579>3.0.CO;2-C

    Article  CAS  Google Scholar 

  11. Schultz CL, Moini M (2003) Anal Chem 75:1508–1513. doi:10.1021/ac0263925

    Article  CAS  Google Scholar 

  12. Soga T, Heiger DN (2000) Anal Chem 72:1236–1241. doi:10.1021/ac990976y

    Article  CAS  Google Scholar 

  13. Soga T, Kakazu Y, Robert M, Tomita M, Nishioka T (2004) Electrophoresis 25:1964–1972. doi:10.1002/elps.200305791

    Article  CAS  Google Scholar 

  14. Mayboroda OA, Neususs C, Pelzing M, Zurek G, Derks R, Meulenbelt I, Kloppenburg M, Slagboom EP, Deelder AM (2007) J Chromatogr A 1159:149–153. doi:10.1016/j.chroma.2007.04.055

    Article  CAS  Google Scholar 

  15. Baidoo EEK, Benke PI, Neususs C, Pelzing M, Kruppa G, Leary JA, Keasling JD (2008) Anal Chem 80:3112–3122. doi:10.1021/ac800007q

    Article  CAS  Google Scholar 

  16. Williams BJ, Cameron CJ, Workman R, Broeckling CD, Sumner LW, Smith JT (2007) Electrophoresis 28:1371–1379. doi:10.1002/elps.200600609

    Article  CAS  Google Scholar 

  17. Lee R, Ptolemy AS, Niewczas L, Britz-McKibbin P (2007) Anal Chem 79:403–415. doi:10.1021/ac061780i

    Article  CAS  Google Scholar 

  18. Pálmarsdóttir S, Mathiasson L, Jönsson JÅ, Edholm LE (1997) J Chromatogr B 688:127–134. doi:10.1016/S0378-4347(97)88064-4

    Article  Google Scholar 

Download references

Acknowledgments

This work was financially supported by the Key Program of Science and Technology of Zhejiang Province (No. 2005C13026).

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Authors and Affiliations

  1. Pharmaceutical Informatics Institute, Zhejiang University, 310058, Hangzhou, China

    Shufang Wang & Ping Yang

  2. College of Preclinical Medicine, Zhejiang Chinese Medical University, 310053, Hangzhou, China

    Xiaoping Zhao

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  1. Shufang Wang
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  2. Ping Yang
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  3. Xiaoping Zhao
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Correspondence to Xiaoping Zhao.

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Wang, S., Yang, P. & Zhao, X. Amino Acid Profile Determination in the Urine of Bladder Cancer Patients by CE-MS with On-Line pH-Mediated Stacking and Pattern Recognition. Chroma 70, 1479–1484 (2009). https://doi.org/10.1365/s10337-009-1319-1

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  • DOI: https://doi.org/10.1365/s10337-009-1319-1

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