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Simultaneous Determination of Anabolic Androgenic Steroids and Their Esters in Hair by LC–MS–MS

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Abstract

A liquid chromatographic-tandem mass spectrometric method for the simultaneous determination of anabolic androgenic steroids and their esters in hair has been developed. The hair sample was treated with methanol to extract the esters, followed by alkaline digestion for optimum recovery of the anabolic androgenic steroids. After liquid–liquid extractions, the extract was dried, redissolved and analyzed by multiple reaction monitoring with a quadrupole mass spectrometer. The lower limits of detection ranged from 0.001 to 0.020 ng mg−1 for the 21 analytes. The applicability of the method was demonstrated using guinea pig hair samples gained from controlled experiments.

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Acknowledgments

The authors wish to thank Dr. Moutian Wu for supplying the IS. This research was supported by the National Natural Science Foundation of China (No. 20475037) and National Institute Scientific program (No. GY0601).

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Authors and Affiliations

  1. Department of Forensic Toxicology, Institute of Forensic Sciences, Ministry of Justice, Shanghai Key Laboratory of Forensic Medicine, Guangfu Xi Road 1347, Shanghai, 200063, China

    Min Shen, Hui Yan, Ping Xiang & Baohua Shen

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  1. Min Shen
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  2. Hui Yan
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  3. Ping Xiang
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  4. Baohua Shen
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Correspondence to Min Shen.

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Shen, M., Yan, H., Xiang, P. et al. Simultaneous Determination of Anabolic Androgenic Steroids and Their Esters in Hair by LC–MS–MS. Chroma 70, 1381–1386 (2009). https://doi.org/10.1365/s10337-009-1314-6

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  • DOI: https://doi.org/10.1365/s10337-009-1314-6

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