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Analysis of Bioactive Saponins in Fructus Gleditsiae abnormalis and Fructus Gleditsiae sinensis by LC-ELSD

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Abstract

Fructus Gleditsiae abnormalis and Fructus Gleditsiae sinensis are widely used in traditional Chinese medicine. As different developmental stages of fruits of the same plant Gleditsia sinensis Lam., whether Fructus Gleditsiae sinensis can be used as the substitute of Fructus Gleditsiae abnormalis has long been debated. To compare the differences of the content of main active saponins between the two fruits, we established a new LC-ELSD method for simultaneous determination of four major saponins present in two fruits. After fully validated, the method was successfully applied to quantitatively analyze the four bioactive saponins of twenty samples, 11 of Fructus Gleditsiae abnormalis and 9 of Fructus Gleditsiae sinensis obtained from different locations. It was shown that there were no significant differences in saponins contents and LC profiles between the two fruits. Moreover, the developed method can be used for quality control of Fructus Gleditsiae abnormalis and Fructus Gleditsiae sinensis.

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Acknowledgments

This work was supported by the “Blue Project” of Jiangsu Province Education Department, the “333 High-Level Personnel Training” and “Hi-Tech Research Programs” of Jiangsu Province.

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Authors and Affiliations

  1. Department of Chinese Materia Medica Analysis, China Pharmaceutical University, 210009, Nanjing, People’s Republic of China

    Yufeng Xia, Zhengzhen Gao & Qiang Wang

  2. Department of Pharmacology of Chinese Materia Medica, China Pharmaceutical University, 210009, Nanjing, People’s Republic of China

    Yue Dai

Authors
  1. Yufeng Xia
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  2. Zhengzhen Gao
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  3. Yue Dai
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  4. Qiang Wang
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Correspondence to Yufeng Xia.

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Xia, Y., Gao, Z., Dai, Y. et al. Analysis of Bioactive Saponins in Fructus Gleditsiae abnormalis and Fructus Gleditsiae sinensis by LC-ELSD. Chroma 70, 1361–1366 (2009). https://doi.org/10.1365/s10337-009-1312-8

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  • DOI: https://doi.org/10.1365/s10337-009-1312-8

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