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Simultaneous Determination of Berberine and Palmatine in Rabbit Plasma by LC-MS–MS and Its Application in Pharmacokinetic Study After Oral Administration of Coptidis and Coptidis–Gardeniae Couple Extract

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Abstract

A valid and sensitive LC-MS–MS method is adopted for pharmacokinetics study of berberine and palmatine in rabbit plasma. After mixing with internal standard tetrahydroberberine, plasma samples were pretreated with 1.5 mL acetonitrile. Chromatographic separation was on a C18 column using a mixture of water (containing 10 mmol L−1 ammonium acetate, pH 3.5) and acetonitrile (50∶50, v/v) as mobile phase. The detection was performed by selected ion monitoring mode via electrospray ionization source operating in the positive ionization mode. The method was linear over the concentration range of 2.0–200.0 ng mL−1 for berberine and 1.0–100.0 ng mL−1 for palmatine. The lowest limits of quantitation (LLOQ) were 2.0 ng mL−1 for berberine and 1.0 ng mL−1 for palmatine. The intra- and inter-day precision values were less than 14.3% and the deviations were within ±11.0%. The fully validated LC-MS–MS method has been successfully applied to a pharmacokinetic study of berberine, palmatine in rabbit plasma after oral administration of Coptidis and coptidis–gardeniae couple extract. The results indicated that the plasma profiles of the two compounds in rabbit confirmed to one-compartment open model and the combinational utilization with Gardeniae could increase the bioavailability of berberine and palmatine, the two major active components of Coptidis.

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References

  1. Wang L, Hong XK (2003) Chin Tradit Pat Med 25:912–914. doi:cnki:ISSN:1001-1528.0.2003-11-017

    Google Scholar 

  2. Li KL, Sheu SJ (1995) Anal Chim Acta 313:113–120. doi:10.1016/0003-2670(95)00190-B

    Article  CAS  Google Scholar 

  3. Wang J, Wang YY, Yang C (2005) Chin J Chin Mater Med 30:6–8. doi:cnki:ISSN:1001-5302.0.2005-01-001

    Google Scholar 

  4. Ou M (1992) Chinese-English manual of common-used traditional medicine. Guangdong Science and Technology Publishing House, Guangzhou

    Google Scholar 

  5. Zhuang P, Huang MY (1994) Chin Tradit Herb Drug 25:425–428. doi:cnki:ISSN:0253-2670.0.1994-08-019

    CAS  Google Scholar 

  6. Yang F, Zhang T, Zhang R, Ito Y (1998) J Chromatogr A 829(1/2):137–141. doi:10.1016/S0021-9673(98)00776-6

    Article  CAS  Google Scholar 

  7. Lin S, Tsai SC, Lee CC, Wang BW, Liou JY, Shyu KG (2004) Mol Pharmacol 66:612–619. doi:0026-895X/04/6603-612-619

    CAS  Google Scholar 

  8. Peng WH, Wu CR, Chen CS, Chen CF, Leu ZC, Hsieh MT (2004) Life Sci 75:2451–2462. doi:10.1016/j.lfs.2004.04.032

    Article  CAS  Google Scholar 

  9. Kuo CL, Chi CW, Liu TY (2004) Cancer Lett 203:127–137. doi:10.1016/j.canlet.2003.09.002

    Article  CAS  Google Scholar 

  10. Higashi Y, Ikeda Y, Yamamoto R, Yamashiro M, Fujii Y (2005) Life Sci 77:1055–1067. doi:10.1016/j.lfs.2005.03.003

    Article  CAS  Google Scholar 

  11. Koitabashi Y, Kumai T, Matsumoto N, Watanabe M, Sekine S, Yanagida Y, Kobayashi S (2006) Life Sci 78:2852–2859. doi:10.1016/j.lfs.2005.11.006

    Article  CAS  Google Scholar 

  12. Baldan N, Rigotti P, Furian L, Margani G, Ekser B, Frison L, Martin SD, Palatini P (2006) Pharmacol Res 54:181–185. doi:10.1016/j.phrs.2006.04.006

    Article  CAS  Google Scholar 

  13. Bergamaschi CDC, Motta RHL, Franco GCN, Cogo K, Montan MF, Ambrosano GMB, Rosalen PL, Del Fiol FDS, Groppo FC (2006) Int J Antimicrob Agents 27:417–422. doi:10.1016/j.ijantimicag.2006.02.005

    Article  CAS  Google Scholar 

  14. Shin SC, Choi JS, Li XG (2006) Int J Pharm 313:144–149. doi:10.1016/j.ijpharm.2006.01.028

    Article  CAS  Google Scholar 

  15. Zuo F, Nakamura N, Akao T, Hattori M (2006) Drug Metab Dispos 34:2064–2072. doi:10.1124/dmd.106.011361

    Article  CAS  Google Scholar 

  16. Yu S, Pang XY, Deng YX, Liu L, Liang Y, Liu XD, Xie L, Wang GJ, Wang XT (2007) Int J Mass Spectrom 268:30–37. doi:10.1016/j.ijms.2007.08.003

    Article  CAS  Google Scholar 

  17. Deng YT, Liao QF, Li SH, Bi KS, Pan BY, Xie ZY (2008) J Chromatogr B 863:195–205. doi:10.1016/j.jchromb.2007.12.028

    Article  CAS  Google Scholar 

  18. Lu T, Liang Y, Song J, Xie L, Wang GJ, Liu XD (2006) J Pharm Biomed Anal 40:1218–1224. doi:10.1016/j.jpba.2005.09.023

    Article  CAS  Google Scholar 

  19. Hua WY, Ding L, Chen Y, Gong B, He JC, Xu GL (2007) J Pharm Biomed Anal 44:931–937. doi:10.1016/j.jpba.2007.03.022

    Article  CAS  Google Scholar 

  20. Hong XK, Wang L, Wang ZH (1999) Acta uni Trad Med Sin Pharmacol Shanghai 13:51–53. doi:cnki:ISSN:1008-861X.0.1999-02-017

    Google Scholar 

  21. US Department of Health and Human Services, Food and Drug Administration, Center for Drug Evaluation and Research (CDER), Guidance for Industry, Bioanalytical Method Validation, May (2001)

  22. Jiang WY (2005) Trends Pharmacol Sci 26:558–563. doi:10.1016/j.tips.2005.09.006

    Article  CAS  Google Scholar 

Download references

Acknowledgment

Financial supports from the National Natural Sciences Foundation of China (No. 20875074,20875075), West Light Foundation of The Chinese Academy of Sciences (No. 2007DF02) and grants from the Administration of Traditional Chinese Medicine of China (No. 207151) and Ministry of Science and Technology of Shaanxi Province (No. 2006kz10-G5).

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Authors and Affiliations

  1. Shanxi Institute for Food and Drug Control, 710061, Xi’an, Shaanxi, People’s Republic of China

    Xia Li, Haijing Liu & Ji Li

  2. Engineering and Research Center of Chinese Herb Modernization, Northwest University, 710069, Xi’an, Shaanxi, People’s Republic of China

    Xinfeng Zhao, Shixiang Wang & Xiaohui Zheng

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  1. Xia Li
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  2. Haijing Liu
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  3. Ji Li
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Correspondence to Xiaohui Zheng.

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Li, X., Liu, H., Li, J. et al. Simultaneous Determination of Berberine and Palmatine in Rabbit Plasma by LC-MS–MS and Its Application in Pharmacokinetic Study After Oral Administration of Coptidis and Coptidis–Gardeniae Couple Extract. Chroma 70, 1113–1119 (2009). https://doi.org/10.1365/s10337-009-1308-4

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